Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 27°C
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
After several dispointment from other commercialized RacGAP1 antibody, finally we found this one as the best for immunofluorescence. Signals are very strong and clean, even with 1000x dilution. Signals are observed in correct localization - midbody and midzone during cytokinesis.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Dec 16 2008