Key features and details
- Goat polyclonal to RACGAP1/MGCRACGAP
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
- Isotype: IgG
参阅全部 RACGAP1/MGCRACGAP 一抗
经测试应用适用于: IHC-P, ICC, WBmore details
预测可用于: Cow, Dog
- WB: Human testis tissue lysate. K562 and Jurkat cell lysates. ICC: MCF7 cells. IHC-P: Human testis tissue.
Principal Names - RACGAP1/MGCRACGAP; Rac GTPase activating protein 1; ID-GAP; MgcRacGAP; GTPase activating protein. Official Gene Symbol - - RACGAP1/MGCRACGAP. GenBank Accession Number – NP_037409. LocusLink ID - 29127.
This product was previously labelled as RACGAP1
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab2270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|ICC||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
1 hour primary incubation is recommended for this product.
功能Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
组织特异性Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
序列相似性Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain.
结构域The coiled coil region is indispensible for localization to the midbody during cytokinesis.
翻译后修饰Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
细胞定位Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
- Information by UniProt
- CYK4 antibody
- GAP antibody
- Gap1 antibody
All lanes :
Lane 1 : K562 cell lysate (in RIPA buffer)
Lane 2 : Jurkat cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 71 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab2270 (1µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer (pH9), HRP-staining.
Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 0.2 µg/ml + Human Testis lysate (RIPA buffer) at 30 µg
Predicted band size: 71 kDa
ab2270 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 1 µg/ml + K562 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab2270 被引用在 23 文献中.
- Khalid M et al. Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation. Cancers (Basel) 11:N/A (2019). PubMed: 30866526
- Pu J et al. lncRNA MAGI2-AS3 Prevents the Development of HCC via Recruiting KDM1A and Promoting H3K4me2 Demethylation of the RACGAP1 Promoter. Mol Ther Nucleic Acids 18:351-362 (2019). PubMed: 31629962
- Adriaans IE et al. PLK1 plays dual roles in centralspindlin regulation during cytokinesis. J Cell Biol 218:1250-1264 (2019). PubMed: 30728176
- Capalbo L et al. The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nat Commun 10:4513 (2019). PubMed: 31586073
- Barbiero I et al. CDKL5 localizes at the centrosome and midbody and is required for faithful cell division. Sci Rep 7:6228 (2017). PubMed: 28740074