为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Immunology Immunoglobulins Heavy Chain IgG

兔抗叙利亚仓鼠IgG H&L (Biotin) (ab6782)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (6)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)

    Key features and details

    • Rabbit Anti-Syrian Hamster IgG H&L (Biotin)
    • Conjugation: Biotin
    • Host species: Rabbit
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB

    Conjugates logo Related conjugates and formulations

    HRP Texas Red ® Unconjugated

    你可能会感兴趣

    偶联
    Product image
    Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
    封闭
    Normal Rabbit Serum (ab166640)
    IHC
    Product image
    Streptavidin HRP (ready-to-use) (ab64269)

    查看更多相关产品

    概述

    • 产品名称

      兔抗叙利亚仓鼠IgG H&L (Biotin)
      参阅全部 IgG 二抗
    • 宿主

      Rabbit
    • 靶标种属

      Syrian hamster
    • 经测试应用

      适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
    • 免疫原

      hamster IgG whole molecule

    • 偶联物

      Biotin

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...
    • 纯度

      Affinity purified
    • 纯化说明

      Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
    • 共轭说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
    • 克隆

      多克隆
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Hamster
      • IgG
      • Biotin

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6782于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Dot blot
    ELISA
    IHC-P
    IHC-Fr
    Immunomicroscopy
    ICC/IF
    WB
    说明
    应用说明
    immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Syrian Hamster IgG H&L (Biotin) (ab6782)
      Ab6782 was used at dilution 1/800 with the primary antibody ab11936 in IHC-P. See the review on ab11936.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab6782?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6782 被引用在 6 文献中.

    • Zhang M  et al. LncRNA SNHG5 affects cell proliferation, metastasis and migration of colorectal cancer through regulating miR-132-3p/CREB5. Cancer Biol Ther 20:524-536 (2019). PubMed: 30395767
    • Lee D  et al. HMGB2 is a novel adipogenic factor that regulates ectopic fat infiltration in skeletal muscles. Sci Rep 8:9601 (2018). PubMed: 29942000
    • Liu J  et al. Downregulation of let-7b promotes COL1A1 and COL1A2 expression in dermis and skin fibroblasts during heat wound repair. Mol Med Rep 13:2683-8 (2016). PubMed: 26861712
    • Alymova IV  et al. A novel cytotoxic sequence contributes to influenza A viral protein PB1-F2 pathogenicity and predisposition to secondary bacterial infection. J Virol 88:503-15 (2014). PubMed: 24173220
    • Guo Q  et al. Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles. Int J Nanomedicine 8:2273-84 (2013). IHC-P . PubMed: 23818783
    • Barnes NL  et al. Cyclooxygenase-2 inhibition: effects on tumour growth, cell cycling and lymphangiogenesis in a xenograft model of breast cancer. Br J Cancer 96:575-82 (2007). PubMed: 17285134

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    Following our standard protocol for IHC, I could not detect specific staining for CD11c in spleen and ear (dermis) sections. A staining with anti MHC II that I performed in parallel worked just fine. Could you please suggest me how to improve my protocol? According to a paper stated in your bibliography, Metlay 1990 J. Exp. Med. 171:1753, I used acetone for fixation. 1. Take (cryo-)sections out the freezer and warm at RT for 30` 2. Fixation: in cuvette with ice-cold acetone 10` 3. Permeabilization and washing: 3 x 5` in PBS at RT, 1st wash with 0.2%TX-100 4. Blocking: 30` with 5% goat serum in PBS (500µl/slide) 5. 1st antibody: in 5% goat serum O/N, 4ºC (200µl/slide + parafilm) I used up to 1:50 dilution for the hamster anti-CD11c (20ng/ul), I controled the staining with sections, that did not receive 1st antibody. Also 1: 10,000 DAPI 6. Washing: 3 x 10` with PBS 7. 2nd antibody: in 2% goat serum O/N, 4ºC (200µl/slide + parafilm), I used your BIOTIN-anti Hamster 1:200 (5ng/ul) 8. Washing: 3 x 10` with PBS 9. ST-TRITC: in 2% goat serum O/N, 4ºC (200µl/slide + parafilm), I used a standard concentration of 1:200, that works well in other stainings 10. Washing: 2 x 10` in PBS 1 x 10` in Tris-HCl, pH 7.4 (remove salt) 11. Mounting: dry slides for a few minutes, Mowiol, store in dark at 4ºC I would be happpy to receive any suggestions,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 01 2006

    Answer

    I apologise for the delay in responding to you, I have at last received feedback from the laboratory that tested ab33483 and include their response below: I have had a look through your customers protocol and note that it differs somewhat from our recommended protocol for cryostat sections. So firstly from their protocols: 1. I note sections appear to be stored in a freezer prior to staining. Some antigens are more sensitive to this than others, this may be the case here. I would recommend using freshly sectioned material 2. Following acetone fixation they have included a triton step (for the DAPI?). This may not be compatible with staining for CD11c using this antibody. I would suggest omitting this step. I note from the literature that such a step is not included in publications reporting successful staining with this antibody. 3. We do not support the use of Immunofluorescence for this antibody. I do not know whether there is sufficient sensitivity to allow the use of TRIT-C for its detection. I would suggest the use of a peroxidase-DAB protocol as has been used in all the publication reporting successful. My gut feeling is that it may be the Triton X-100 that may affect the epitope recognised by the antibody. Just omitting this step may help restore antigenicity. Please find a list of publications where this has been used successfully for IHC on cryostat sections. There may be further appropriate advice in these. 1: Witmer-Pack MD, Crowley MT, Inaba K, Steinman RM. Macrophages, but not dendritic cells, accumulate colloidal carbon following administration in situ. J Cell Sci. 1993 Aug;105 ( Pt 4):965-73. PMID: 7693737 2: Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71. PMID: 2185332 3: McMenamin PG. Dendritic cells and macrophages in the uveal tract of the normal mouse eye. Br J Ophthalmol. 1999 May;83(5):598-604. PMID: 10216062 4: Pimorady-Esfahani A, Grounds MD, McMenamin PG. Macrophages and dendritic cells in normal and regenerating murine skeletal muscle. Muscle Nerve. 1997 Feb;20(2):158-66. PMID: 9040653 5: Kelsall BL, Strober W. Distinct populations of dendritic cells are present in the subepithelial dome and T cell regions of the murine Peyer's patch. J Exp Med. 1996 Jan 1;183(1):237-47. PMID: 8551227 6: Dahlen E, Dawe K, Ohlsson L, Hedlund G. Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse. J Immunol. 1998 Apr 1;160(7):3585-93. PMID: 9531322 7: Leenen PJ, Radosevic K, Voerman JS, Salomon B, van Rooijen N, Klatzmann D, van Ewijk W. Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover. J Immunol. 1998 Mar 1;160(5):2166-73. PMID: 9498754 8: Gonzalez-Juarrero M, Orme IM. Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis. Infect Immun. 2001 Feb;69(2):1127-33. PMID: 11160010 9: Hamada H, Hiroi T, Nishiyama Y, Takahashi H, Masunaga Y, Hachimura S, Kaminogawa S, Takahashi-Iwanaga H, Iwanaga T, Kiyono H, Yamamoto H, Ishikawa H. Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine. J Immunol. 2002 Jan 1;168(1):57-64. PMID: 11751946 10: Bjorck P. Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity. J Immunol. 2004 May 1;172(9):5396-404. PMID: 15100280 Please note that most of these appear to be available freely via PubMed. Reference 1 has some great pictures of staining in the liver and in spleen with N418 and other antibodies such as F4/80 and FA-11 etc. I hope this advice will be useful. Please do not hesitate to contact me if you still experience problems with those recommendations, if you purchased the antibody in the last 90 days I would be happy to offer you a refund,

    Read More

    Abcam Scientific Support

    回复于 Sep 18 2006

    Question

    I found this information on the web: http://www.histosearch.com/histonet/Oct04/Re.HistonetBackgroundwithC.html where it is recommended to pre-absorb the secondary detecting a hamster monoclonal to CD11c. I bought both antibodies from you, please advise on how to use the secondary. Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2006

    Answer

    Thank you for your enquiry. The protocol quoted in the link you kindly provided does not seem to mention our antibodies but antibodies from other companies. In our experience the secondary ab6782 does not give background problems and does not need to be pre-absorbed. If you experience any problems or need further advice regarding your IHC protocol please do not hesitate to contact me, I would be happy to look at your full protocol,

    Read More

    Abcam Scientific Support

    回复于 Aug 31 2006

    Question

    Is this Ab appropriate as a labeling secondary Ab (for IHC) to an Armenian hamster IgG, group 3, lamda (anti-mouse CD51 monoclonal antibody)

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2001

    Answer

    This antibody is not recommended for Armenian hamster IgG.

    Read More

    Neil Hayward

    Abcam Scientific Support

    回复于 Jul 10 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2022 艾博抗(上海)贸易有限公司版权所有