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兔抗羊IgG H&L (HRP) (ab6747)

Reviews (1)Q&A (2)References (45)
Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

Key features and details

  • Rabbit Anti-Sheep IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin FITC Glucose Oxidase Gold 10nm Gold 15nm Texas Red ® TRITC Unconjugated Unconjugated

概述

  • 产品名称

    兔抗羊IgG H&L (HRP)
    参阅全部 IgG 二抗

  • 宿主

    Rabbit

  • 靶标种属

    Sheep

  • 经测试应用

    适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details

  • 免疫原

    Sheep IgG whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • 克隆

    多克隆

  • 同种型

    IgG

  • 常规说明

    Horseradish Peroxidase (HRP).

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6747于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
WB (1)
1/1000 - 1/4000. PubMed: 15319485
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-Fr
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.
WB
1/1000 - 1/4000. PubMed: 15319485
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-Fr
Use at an assay dependent concentration.

图片

  • Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

    ab6747 was used at dilution 1/2000 with the primary antibody ab7832 in WB. See the review on ab7832.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)Kälble F et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016).

    Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar: 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
    Ab6747 was used at dilution 1/100 with the primary antibody ab53842 in IHC-P. See the review on ab53842.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (45)

发表研究结果有使用 ab6747？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6747 被引用在 45 文献中.

  • Li J  et al. Overexpression of PFKFB3 promotes cell glycolysis and proliferation in renal cell carcinoma. BMC Cancer 22:83 (2022). PubMed: 35057732
  • Cao L  et al. Yes-associated protein expression is associated with poor prognosis in patients with colorectal cancer. Oncol Lett 22:642 (2021). PubMed: 34386064
  • Komarova EY  et al. Hsp70-containing extracellular vesicles are capable of activating of adaptive immunity in models of mouse melanoma and colon carcinoma. Sci Rep 11:21314 (2021). PubMed: 34716378
  • Buzzo JR  et al. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix. Cell 184:5740-5758.e17 (2021). PubMed: 34735796
  • Evert BJ  et al. Epitope-coated polymer particles elicit neutralising antibodies against Plasmodium falciparum sporozoites. NPJ Vaccines 6:141 (2021). PubMed: 34845267
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

A good 2nd antibody for WB

 Excellent
Abreviews
Application
Western blot
This is a good 2nd antibody for WB assay.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 24 2020

Question

Estimado/a Sr/Sra:

Me pongo en contacto con usted porque estoy interesada en tener los certificados de análisis de los siguientes productos que hemos adquirido de Abcam:

-hTF Sheep polyclonal Ab (Ab61372), batch: XXXXX
-anti- sheep Ab (Ab6747), batch: XXXXX

Por favor, le rogaría me lo pudiese proporcionar lo antes posible.

Un cordial saludo

María

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Answer

Gracias por contactarnos.

Lamentablemente no enviamos Certificados de Análisis de ninguno de nuestros productos.

Lo que si podemos enviaros, y los adjunto a este mail, son Certificados de Conformidad.

Espero que os sea de utilidad, y en cualquier caso no dudéis en contactarnos para cualquier duda o sugerencia.

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Question

Dear Sir



The customer would like to get the certification of ab53586 .lot.GR10254 and ab6747 lot 948846.



Pleases kindly send to me.





Best regards

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Answer

Thank you for your inquiry.

Please find attached the Certificates of Compliance for ab6747 and ab53586.

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