兔抗羊IgG H&L (HRP) (ab6747)
Key features and details
- Rabbit Anti-Sheep IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr
Related conjugates and formulations
概述
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产品名称
兔抗羊IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Sheep -
经测试应用
适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details -
免疫原
Sheep IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
克隆
多克隆 -
同种型
IgG -
常规说明
Horseradish Peroxidase (HRP).
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研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/1000 - 1/4000. PubMed: 15319485
|
| ELISA |
Use at an assay dependent dilution.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500 - 1/2500.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/4000. PubMed: 15319485 |
|
ELISA
Use at an assay dependent dilution. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ICC/IF
1/500 - 1/2500. |
|
IHC-Fr
Use at an assay dependent concentration. |
图片
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Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)Kälble F et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016).Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar: 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab6747 被引用在 31 文献中.
- Fu D et al. Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system. BMC Musculoskelet Disord 21:281 (2020). PubMed: 32359349
- Zhao X et al. CREB1-induced miR-1204 promoted malignant phenotype of glioblastoma through targeting NR3C2. Cancer Cell Int 20:111 (2020). PubMed: 32280303
- Huang Y et al. Function of miR-200a in proliferation and apoptosis of non-small cell lung cancer cells. Oncol Lett 20:1256-1262 (2020). PubMed: 32724366
- Young SJ et al. MutSß Stimulates Holliday Junction Resolution by the SMX Complex. Cell Rep 33:108289 (2020). PubMed: 33086055
- Xiao Z et al. Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation. Oncol Rep 41:2667-2678 (2019). PubMed: 30896884