兔抗羊IgG H&L (Glucose Oxidase) (ab136721)
Key features and details
- Rabbit Anti-Sheep IgG H&L (Glucose Oxidase)
- Conjugation: Glucose Oxidase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, IHC-Fr, ELISA, ICC, WB
Related conjugates and formulations
概述
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产品名称
兔抗羊IgG H&L (Glucose Oxidase)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Sheep -
经测试应用
适用于: IHC-P, IHC-Fr, ELISA, ICC, WBmore details -
免疫原
Highly purified whole immunoglobulin from Sheep.
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偶联物
Glucose Oxidase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab136721 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
克隆
多克隆 -
同种型
IgG -
常规说明
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab136721于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/10 - 1/100.
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|
IHC-Fr |
1/10 - 1/100.
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|
ELISA |
1/100 - 1/1000.
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|
ICC |
1/10 - 1/100.
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|
WB |
1/400.
|
说明 |
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IHC-P
1/10 - 1/100. |
IHC-Fr
1/10 - 1/100. |
ELISA
1/100 - 1/1000. |
ICC
1/10 - 1/100. |
WB
1/400. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab136721 尚未被引用在任何文献中。