兔抗大鼠IgG H&L (HRP) (ab6734)
Key features and details
- Rabbit Anti-Rat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr
概述
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产品名称
兔抗大鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Rat -
经测试应用
适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details -
免疫原
Rat IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Horseradish Peroxidase (HRP) -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
|
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WB | (1) |
1/1000 - 1/5000.
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ELISA |
1/60000.
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|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/500 - 1/2500.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000 - 1/5000. |
ELISA
1/60000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/2500. |
IHC-Fr
Use at an assay dependent concentration. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.
ab25377 staining Ly6g in mouse placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS/1% BSA) for 12 hours at 4°C. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/200) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)This image is courtesy of an anonymous Abreview.
ab6326 staining BrdU in mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with protein blocking solution for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) in Ab diluent for 1 hour. A HRP-conjugated rabbit anti-rat IgG H&L (ab6734) (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Rat IgG H&L (HRP) (ab6734)
IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6734, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (61)
ab6734 被引用在 61 文献中.
- Zhu N et al. Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis In Vitro and Myocardial Ischemia/Reperfusion-Induced Apoptosis In Vivo by Regulating the Mitophagy-Mediated HIF-1a/BNIP3 Pathway. Front Pharmacol 11:367 (2020). PubMed: 32292345
- Lagree K et al. Roles of Candida albicans Mig1 and Mig2 in glucose repression, pathogenicity traits, and SNF1 essentiality. PLoS Genet 16:e1008582 (2020). PubMed: 31961865
- Potilinski MC et al. Elucidating the mechanism of action of alpha-1-antitrypsin using retinal pigment epithelium cells exposed to high glucose. Potential use in diabetic retinopathy. PLoS One 15:e0228895 (2020). PubMed: 32032388
- Padthaisong S et al. Overexpression of a panel of cancer stem cell markers enhances the predictive capability of the progression and recurrence in the early stage cholangiocarcinoma. J Transl Med 18:64 (2020). PubMed: 32039729
- Trus I et al. Zika Virus with Increased CpG Dinucleotide Frequencies Shows Oncolytic Activity in Glioblastoma Stem Cells. Viruses 12:N/A (2020). PubMed: 32466170