兔抗大鼠IgG H&L (HRP) (ab102172)
Key features and details
- Rabbit Anti-Rat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
概述
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产品名称
兔抗大鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Rat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
经测试应用
适用于: IHC-P, ICC, ELISA, WBmore details -
偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP. -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102172于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/200 - 1/5000.
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|
ICC |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. (as primary)
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WB |
1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).
|
说明 |
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IHC-P
1/200 - 1/5000. |
ICC
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (as primary) |
WB
1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000). |
图片
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Lane 1 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
Lanes 2-6 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/2000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/10000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lane 5 : Goat polyclonal to RUNX2 (ab102712) at 1/20000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lane 6 : Rabbit Anti-Rat IgG H&L (HRP) (ab102172) at 1/20000 dilution (Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 52 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab102172 被引用在 3 文献中.
- Bochnakian A et al. Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA. J Virol 93:N/A (2019). PubMed: 31341054
- Fung L et al. miR-128 Restriction of LINE-1 (L1) Retrotransposition Is Dependent on Targeting hnRNPA1 mRNA. Int J Mol Sci 20:N/A (2019). PubMed: 31010097
- Idica A et al. MicroRNA miR-128 represses LINE-1 (L1) retrotransposition by down-regulating the nuclear import factor TNPO1. J Biol Chem 292:20494-20508 (2017). PubMed: 28974576