兔抗大鼠IgG H&L (FITC) (ab6730)

Reviews (1)Q&A (2)References (11)
Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)

    Key features and details

    • Rabbit Anti-Rat IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Rabbit
    • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    概述

    • 产品名称

      兔抗大鼠IgG H&L (FITC)
      参阅全部 IgG 二抗

    • 宿主

      Rabbit

    • 靶标种属

      Rat

    • 经测试应用

      适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

    • 免疫原

      Rat whole molecule

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 共轭说明

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

    • 克隆

      多克隆

    • 研究领域

    相关产品

      应用

      The Abpromise guarantee

      Abpromise™承诺保证使用ab6730于以下的经测试应用

      “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

      应用 Ab评论 说明
      Immunomicroscopy
      Use at an assay dependent concentration.
      Flow Cyt
      Use at an assay dependent dilution.
      ICC/IF (1)
      1/1000 - 1/5000.
      IHC-P
      Use at an assay dependent concentration.
      IHC-Fr
      Use at an assay dependent concentration.
      ELISA
      1/10000 - 1/50000.
      说明
      Immunomicroscopy
      Use at an assay dependent concentration.
      Flow Cyt
      Use at an assay dependent dilution.
      ICC/IF
      1/1000 - 1/5000.
      IHC-P
      Use at an assay dependent concentration.
      IHC-Fr
      Use at an assay dependent concentration.
      ELISA
      1/10000 - 1/50000.

      图片

      • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)
        Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)
        Ab6730 was used at dilution 1/500 with the primary antibody ab53444 in IHC-Fr. See the review on ab53444.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (11)

      发表研究结果有使用 ab6730？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6730 被引用在 11 文献中.

      • Zhu L  et al. Ampelopsin-sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro. Oncol Lett 18:189-196 (2019). PubMed: 31289488
      • Xu Y  et al. Differentiation of CD45-/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43:1128-1138 (2019). PubMed: 30628669
      • Kneppers A  et al. A novel in vitro model for the assessment of postnatal myonuclear accretion. Skelet Muscle 8:4 (2018). PubMed: 29444710
      • Gholinejad M  et al. Adenosine decreases oxidative stress and protects H2O2-treated neural stem cells against apoptosis through decreasing Mst1 expression. Biomed Rep 8:439-446 (2018). PubMed: 29732147
      • Wan B  et al. Topotecan alleviates ventilator-induced lung injury via NF-?B pathway inhibition. Cytokine N/A:N/A (2018). PubMed: 29803659
      View all Publications for this product

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Secondary ab

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      It works good with abcam as well as other vendors antibodies

      Abcam user community

      Verified customer

      提交于 Sep 05 2014

      Question

      I'm actually looking for an isotype control that would fit with the ab15684(smooth muscle cells antibody HM19/2). Ideally, I would like to use a rat IgM.Do you have any suggestions? Would a rat IgG be a good isotype for immunofluorescence with that antibody? The same question can be apply for the secondary antibody. The technical data sheet suggest ab6730 which is a rabbit polyclonal to rat IgG coupled to a FITC molecule. Is it a good choice?

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      Answer

      Thank you for your recent enquiry. For immunohistochemistry, I would recommend simply omitting the primary antibody step as a control experiment. Indeed any of the secondary antibodies recommended on the datasheet of ab15684 will work (in my view Cy5 might be brighter than FITC and an amplification with HRP or biotin can also enhance greatly your signal if you suspect low abundance of the protein in your samples). You have not mentioned the source of your cells, but please remember the antibody is a rat monoclonal so it cannot be used on rat cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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      Question

      Hi, I am using Rat monoclonal (YOL1/34) to alpha Tubulin (ab9267), dispatched on: 6-Aug-2003 and Rabbit polyclonal to Rat IgG H&L (FITC) (ab6730, dispatched on: 6-Aug-2003. I would like to visualize Plant (Arabidopsis) alpha tubulin. Did any of your customers already try these antibodies in planta?

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      Answer

      We have had no feedback from customers that may have used these antibodies in plant.

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