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Immunology Immunoglobulins Heavy Chain IgG

兔抗大鼠IgG H&L (FITC) (ab6730)

  • Datasheet
Reviews (1)Q&A (2)References (14)

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Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)

    Key features and details

    • Rabbit Anti-Rat IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Rabbit
    • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    Conjugates logo Related conjugates and formulations

    Alkaline Phosphatase beta-galactosidase Biotin HRP Unconjugated Unconjugated

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    概述

    • 产品名称

      兔抗大鼠IgG H&L (FITC)
      参阅全部 IgG 二抗
    • 宿主

      Rabbit
    • 靶标种属

      Rat
    • 经测试应用

      适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
    • 免疫原

      Rat whole molecule

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...
    • 纯度

      Affinity purified
    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    • 共轭说明

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
    • 克隆

      多克隆
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rat
      • IgG
      • Fluorophore
      • FITC

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6730于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF (1)
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    说明
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.

    图片

    • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)
      Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (FITC) (ab6730)
      Ab6730 was used at dilution 1/500 with the primary antibody ab53444 in IHC-Fr. See the review on ab53444.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab6730?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6730 被引用在 14 文献中.

    • Zuo Q  et al. BMP4 activates the Wnt-Lin28A-Blimp1-Wnt pathway to promote primordial germ cell formation via altering H3K4me2. J Cell Sci 134:N/A (2021). PubMed: 33443086
    • Tomczyk-Warunek A  et al. The effect of maternal HMB supplementation on bone mechanical and geometrical properties, as well as histomorphometry and immunolocalization of VEGF, TIMP2, MMP13, BMP2 in the bone and cartilage tissue of the humerus of their newborn piglets. PLoS One 16:e0240642 (2021). PubMed: 33626093
    • Eakin AJ  et al. Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function. Front Cell Dev Biol 8:799 (2020). PubMed: 32974345
    • Zhu L  et al. Ampelopsin-sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro. Oncol Lett 18:189-196 (2019). PubMed: 31289488
    • Xu Y  et al. Differentiation of CD45-/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43:1128-1138 (2019). PubMed: 30628669
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Secondary ab

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    It works good with abcam as well as other vendors antibodies

    Abcam user community

    Verified customer

    提交于 Sep 05 2014

    Question

    I'm actually looking for an isotype control that would fit with the ab15684(smooth muscle cells antibody HM19/2). Ideally, I would like to use a rat IgM.Do you have any suggestions? Would a rat IgG be a good isotype for immunofluorescence with that antibody? The same question can be apply for the secondary antibody. The technical data sheet suggest ab6730 which is a rabbit polyclonal to rat IgG coupled to a FITC molecule. Is it a good choice?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 03 2004

    Answer

    Thank you for your recent enquiry. For immunohistochemistry, I would recommend simply omitting the primary antibody step as a control experiment. Indeed any of the secondary antibodies recommended on the datasheet of ab15684 will work (in my view Cy5 might be brighter than FITC and an amplification with HRP or biotin can also enhance greatly your signal if you suspect low abundance of the protein in your samples). You have not mentioned the source of your cells, but please remember the antibody is a rat monoclonal so it cannot be used on rat cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

    Read More

    Abcam Scientific Support

    回复于 Dec 06 2004

    Question

    Hi, I am using Rat monoclonal (YOL1/34) to alpha Tubulin (ab9267), dispatched on: 6-Aug-2003 and Rabbit polyclonal to Rat IgG H&L (FITC) (ab6730, dispatched on: 6-Aug-2003. I would like to visualize Plant (Arabidopsis) alpha tubulin. Did any of your customers already try these antibodies in planta?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    We have had no feedback from customers that may have used these antibodies in plant.

    Read More

    Asdf Edo

    Abcam Scientific Support

    回复于 Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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