兔抗大鼠IgG H&L (Biotin) (ab6733)
Key features and details
- Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
概述
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产品名称
兔抗大鼠IgG H&L (Biotin)
参阅全部 IgG 二抗 -
描述
兔多克隆抗体二抗to大鼠IgG - H&L (Biotin) -
宿主
Rabbit -
靶标种属
Rat -
经测试应用
适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rat IgG whole molecule
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偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
Our Abpromise guarantee covers the use of ab6733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. | |
| ELISA | 1/500000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
图片
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Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)This image is courtesy of an Abreview submitted by Dr Jiqiang ZhangAb6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.
Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400. -
Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (15)
ab6733 被引用在 15 文献中.
- Lin X et al. Combined a-programmed death-1 monoclonal antibody blockade and fractionated radiation therapy reduces tumor growth in mouse EL4 lymphoma. Cancer Biol Ther 20:666-679 (2019). PubMed: 30572778
- Kasahara K et al. Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model. Nat Microbiol 3:1461-1471 (2018). PubMed: 30397344
- Murakami Y et al. A novel mother-to-child human T-cell leukaemia virus type 1 (HTLV-1) transmission model for investigating the role of maternal anti-HTLV-1 antibodies using orally infected mother rats. J Gen Virol 98:835-846 (2017). PubMed: 28150581
- Xiao R & Chen R Neutrophil gelatinase-associated lipocalin as a potential novel biomarker for ventilator-associated lung injury. Mol Med Rep 15:3535-3540 (2017). PubMed: 28393227
- Dinh CH et al. Bardoxolone Methyl Prevents High-Fat Diet-Induced Colon Inflammation in Mice. J Histochem Cytochem 64:237-55 (2016). PubMed: 26920068
