兔抗大鼠IgG H&L (Biotin) (ab6733)
Key features and details
- Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
概述
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产品名称
兔抗大鼠IgG H&L (Biotin)
参阅全部 IgG 二抗 -
描述
兔多克隆抗体二抗to大鼠IgG - H&L (Biotin) -
宿主
Rabbit -
靶标种属
Rat -
经测试应用
适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rat IgG whole molecule
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偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent dilution.
|
|
| ELISA |
1/500000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent dilution. |
|
ELISA
1/500000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Dot blot
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
图片
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Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)This image is courtesy of an Abreview submitted by Dr Jiqiang ZhangAb6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.
Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400. -
Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab6733 被引用在 16 文献中.
- Nowwarote N et al. Transcriptome analysis of basic fibroblast growth factor treated stem cells isolated from human exfoliated deciduous teeth. Heliyon 6:e04246 (2020). PubMed: 32617420
- Lin X et al. Combined a-programmed death-1 monoclonal antibody blockade and fractionated radiation therapy reduces tumor growth in mouse EL4 lymphoma. Cancer Biol Ther 20:666-679 (2019). PubMed: 30572778
- Kasahara K et al. Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model. Nat Microbiol 3:1461-1471 (2018). PubMed: 30397344
- Murakami Y et al. A novel mother-to-child human T-cell leukaemia virus type 1 (HTLV-1) transmission model for investigating the role of maternal anti-HTLV-1 antibodies using orally infected mother rats. J Gen Virol 98:835-846 (2017). PubMed: 28150581
- Xiao R & Chen R Neutrophil gelatinase-associated lipocalin as a potential novel biomarker for ventilator-associated lung injury. Mol Med Rep 15:3535-3540 (2017). PubMed: 28393227