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Immunology Immunoglobulins Heavy Chain IgG

兔抗大鼠IgG H&L (Biotin) (ab6733)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (15)

Product price, shipping and contact information

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Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)

Key features and details

  • Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

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概述

  • 产品名称

    兔抗大鼠IgG H&L (Biotin)
    参阅全部 IgG 二抗
  • 描述

    兔多克隆抗体二抗to大鼠IgG - H&L (Biotin)
  • 宿主

    Rabbit
  • 靶标种属

    Rat
  • 经测试应用

    适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • 免疫原

    Rat IgG whole molecule

  • 偶联物

    Biotin

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Biotin

相关产品

    应用

    Our Abpromise guarantee covers the use of ab6733 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution.
    ELISA 1/500000.
    Immunomicroscopy Use at an assay dependent concentration.
    Dot blot Use at an assay dependent concentration.
    ICC/IF 1/1000 - 1/5000.
    IHC-P Use at an assay dependent concentration.
    IHC-Fr Use at an assay dependent concentration.

    图片

    • Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
      Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
      Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

      Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
      Immunohistochemistry (Frozen sections) - Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)
      Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (15)

    发表研究结果有使用 ab6733?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6733 被引用在 15 文献中.

    • Lin X  et al. Combined a-programmed death-1 monoclonal antibody blockade and fractionated radiation therapy reduces tumor growth in mouse EL4 lymphoma. Cancer Biol Ther 20:666-679 (2019). PubMed: 30572778
    • Kasahara K  et al. Interactions between Roseburia intestinalis and diet modulate atherogenesis in a murine model. Nat Microbiol 3:1461-1471 (2018). PubMed: 30397344
    • Murakami Y  et al. A novel mother-to-child human T-cell leukaemia virus type 1 (HTLV-1) transmission model for investigating the role of maternal anti-HTLV-1 antibodies using orally infected mother rats. J Gen Virol 98:835-846 (2017). PubMed: 28150581
    • Xiao R & Chen R Neutrophil gelatinase-associated lipocalin as a potential novel biomarker for ventilator-associated lung injury. Mol Med Rep 15:3535-3540 (2017). PubMed: 28393227
    • Dinh CH  et al. Bardoxolone Methyl Prevents High-Fat Diet-Induced Colon Inflammation in Mice. J Histochem Cytochem 64:237-55 (2016). PubMed: 26920068
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    background with ab6733 on Mouse tissue samples

    Read More

    Abcam community

    Verified customer

    Asked on Oct 09 2012

    Answer

    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit ab6733 que vous avez reçu ne fonctionne pas comme attendu.
    Comme convenu j'ai mis en place l'envoi d'une unité de ab7103 (https://www.abcam.com/ab7103), le numéro de commande de remplacement gratuit de ce produit est *****.
    Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

    Read More

    Abcam Scientific Support

    回复于 Oct 09 2012

    Question

    Customer kindly requested Biotin conjugated secondary antibodies for ab2378, ab5690, ab103573, ab10558, ab955, ab53444, ab9018

    Read More

    Abcam community

    Verified customer

    Asked on Aug 15 2011

    Answer

    Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044.html ab5690, ab103573, ab10558 are rabbit polyclonal antibodies. I would recommend using ab6720; https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6720.html ab53444 is a rat monoclonal antibody. I would recommend using ab6733; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6733.html To let you know we are running a promotion '4 for 3' on primaries; if you could select 1 more primary antibody then you will be entitled to 8 primary antibodies for the price of 6. Use the discount code and follow the instructions given on website. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Aug 15 2011

    Question

    Is this or any other anti-rat Ab absorbed to prevent cross reactivity with mouse Abs ? I need to run a double label withj mouse and rat Abs and I need to prevent any cross reactivity. I can't use any rabbit Abs for another technical reason. Thanks David

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2002

    Answer

    I recommend ab7096 -- Goat polyclonal to rat IgG H&L (biotin) Pre-Adsorbed

    Read More

    Danielle Miller

    Abcam Scientific Support

    回复于 Feb 28 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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