兔抗猪IgG H&L (FITC) (ab6773)

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Key features and details

  • Rabbit Anti-Pig IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rabbit
  • Suitable for: Flow Cyt, ICC/IF, ELISA

概述

  • 产品名称

    兔抗猪IgG H&L (FITC)
    参阅全部 IgG 二抗

  • 宿主

    Rabbit

  • 靶标种属

    Pig

  • 经测试应用

    适用于: Flow Cyt, ICC/IF, ELISAmore details

  • 免疫原

    pig whole molecule

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.164% Sodium phosphate, 0.878% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • 共轭说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 克隆

    多克隆

  • 研究领域

相关产品

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6773于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    1/500 - 1/2500.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/10000 - 1/50000.
    说明
    Flow Cyt
    1/500 - 1/2500.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/10000 - 1/50000.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab6773？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6773 被引用在 1 文献中.

    • Simões M  et al. Alterations of Nuclear Architecture and Epigenetic Signatures during African Swine Fever Virus Infection. Viruses 7:4978-96 (2015). PubMed: 26389938

    客户评价及客户问答

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    Question

    Good afternoon,

    we would like to ask for help regarding the next secondary antibodies, guiding me through the choice to use some antibodies for Immunofluorescence in combined forms.

    The target cells are VERO, infected with a porcine virus.

    Some of the primary antibodies have then to be tagged with a goat anti-mouse Cy3 or a donkey anti-mouse FITC.

    To this primary anti-mouse marking we perform another signaling mark with a rabbit anti-pig (ab6775 - already in our lab) or one of the following items:

    rabbit a-pig ab6773 (FITC)
    goat a-pig ab6911 (FITC), Which should result better? Should we be careful about the Fc portion in such combinations?

    To add up to this confusing situation we also have a need for a anti-rabbit Dylight 594. Should we prefer a donkey anti-rabbit (ab 96921 or 96893) or,
    a goat anti-rabbit (ab 96885, 96897 or 98473)?

    Could it be possible to endorse the Invoice proforma of the recommended anti-pig FITC and anti-rabbit Dylight 594 antibodies to:

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    Answer

    Thank you very much for your inquiry and your interest in our products.

    If performing a double or triple staining, it is very important to make all the controls that the secondary antibodies do not crossreact with the immunoglobulins from the other species. For example for the anti pig antibody, it would be important that the anti pig antibody does not recognise the immunoglobulins from mouse or rabbit, not to have a false collocatisation. Unfortunately, non of our anti pig antibodies have been tested for this. However as you have used the ab6775 already successfully in your laboratory if I understood correctly, I can recommend to use the ab6773 as it is from the same original pool. Please do however perform the single color controls to verify the crossreactivity of the ab6773 with rabbit or mouse immunoglobulins.

    In regards to the anti rabbit antibody, I can recommend to use the ab98500, a donkey anti rabbit IgG which has been preabsorbed against mouse, rat and human immunoglobulins, sothere is nocrossreaction against those immunoglobulins. We do not have an anti rabbit DyLight 594 which has been crossabsorbed also against the pig immunoglobulins. If you are concerned or if you have troubles with background staining on the VERO cells, I can recommend to use the same antibody , however with no Fc region, i.e. the F(ab')2 portion. This would be the ab98490.
    https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-594-preadsorbed-ab98500.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-594-preadsorbed-ab98500.html).
    https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLightreg-594-pre-adsorbed-ab98490.html (or use the following: https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-DyLightreg-594-pre-adsorbed-ab98490.html).
    Please let us know which one you would prefer, so we can send the correctproforma invoice.

    The species in which the secondary antibody is generated is not very important. It is only important if you get background staining from the secondary antibody alone, in which case you can then block with serum of that species. However this is not an issue in general, especially not in cell culture.

    I hope this information is helpful.

    We will send theproforma invoices tomorrow also to your email address. I hope this is how you would like to receive it. Please let me know however whether you would like us to send it to another email address or fax.

    Thank you very much. Please do not hesitate to contact us for any further question or advice.

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