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Immunology Immunoglobulins Heavy Chain IgG

兔抗小鼠IgG+IgM+IgA H&L (FITC) (ab8517)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (10)

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Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)

    Key features and details

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Rabbit
    • Suitable for: ICC/IF, IHC-P

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    Normal Rabbit Serum (ab166640)

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    概述

    • 产品名称

      兔抗小鼠IgG+IgM+IgA H&L (FITC)
      参阅全部 IgG + IgM + IgA 二抗
    • 宿主

      Rabbit
    • 靶标种属

      Mouse
    • 特异性

      Antibodies were raised in rabbit to mouse IgG, IgM and IgA
    • 经测试应用

      适用于: ICC/IF, IHC-Pmore details
    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      pH: 7.50
      Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound.
    • 共轭说明

      The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
    • 克隆

      多克隆
    • 常规说明

      F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies
    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgA
      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgM
      • Secondary antibodies
      • anti-Mouse
      • IgG + IgM + IgA
      • Fluorophore
      • FITC

    相关产品

      应用

      The Abpromise guarantee

      Abpromise™承诺保证使用ab8517于以下的经测试应用

      “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

      应用 Ab评论 说明
      ICC/IF
      Use at an assay dependent dilution. PubMed: 15483223
      IHC-P
      Use at an assay dependent dilution.
      说明
      ICC/IF
      Use at an assay dependent dilution. PubMed: 15483223
      IHC-P
      Use at an assay dependent dilution.

      图片

      • Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
        Immunocytochemistry - Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
        Ab8517 was used at dilution 1/200 with the primary antibody ab18230 in ICC/IF. See the review on ab18230.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (10)

      发表研究结果有使用 ab8517?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab8517 被引用在 10 文献中.

      • Zhao M  et al. The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells. Cell Prolif 53:e12758 (2020). PubMed: 31922317
      • Akyash F  et al. Derivation of new human embryonic stem cell lines (Yazd1-3) and their vitrification using Cryotech and Cryowin tools: A lab resources report. Int J Reprod Biomed (Yazd) 17:891-906 (2019). PubMed: 31970311
      • Kolodziej M  et al. First molecular characterization of Dobrava-Belgrade virus found in Apodemus flavicollis in Poland. Ann Agric Environ Med 25:368-373 (2018). PubMed: 29936795
      • Ahn J  et al. Transplantation of human Wharton's jelly-derived mesenchymal stem cells highly expressing TGFß receptors in a rabbit model of disc degeneration. Stem Cell Res Ther 6:190 (2015). IHC ; Human . PubMed: 26432097
      • Michalski A  et al. Surveillance of hantaviruses in Poland: a study of animal reservoirs and human hantavirus disease in Subcarpathia. Vector Borne Zoonotic Dis 14:514-22 (2014). PubMed: 24902039
      • Guo Z  et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol 190:4337-48 (2013). PubMed: 23514739
      • Tang L  et al. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol 226:832-42 (2011). IHC-P . PubMed: 20857425
      • Huang CY  et al. Plasticity of stem cells derived from adult periodontal ligament. Regen Med 4:809-21 (2009). PubMed: 19903001
      • Cai L  et al. Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy. J Am Coll Cardiol 48:1688-97 (2006). PubMed: 17045908
      • Pfister-Genskow M  et al. Identification of differentially expressed genes in individual bovine preimplantation embryos produced by nuclear transfer: improper reprogramming of genes required for development. Biol Reprod 72:546-55 (2005). ICC/IF ; Mouse . PubMed: 15483223

      客户评价及客户问答

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      Question

      compatible secondary. Flow cytometry. Does it necessarily have to be anti- IgM like ab8517?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 15 2010

      Answer

      When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG- they react with light chains, and therefore also react with other immunoglobulin classes (IgA, IgM, IgD, and IgE) and subclasses sharing the same light chains. Other options anti-IgM compatible secondaries besides ab8517 for Flow Cytometry are: ab6785 Goat polyclonal to Mouse IgG - H&L (FITC) ab60313 Goat polyclonal to Mouse IgG (Chromeo™ 488) ab60316 Goat polyclonal to Mouse IgG - H&L (Chromeo™ 546) ab60318 Goat polyclonal to Mouse IgG (Chromeo™ 642) ab6563 and ab7002 are other options. Please bear in mind that the affinity might be lower for IgG H&L antibodies against IgM, therefore the safest would be to use a secondary against IgM.

      Read More

      Abcam Scientific Support

      回复于 Jun 15 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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