兔抗小鼠IgG H&L (HRP) (ab6728)
Key features and details
- Rabbit Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB
Related conjugates and formulations
概述
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产品名称
兔抗小鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
经测试应用
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details -
免疫原
Mouse IgG whole molecule
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. -
共轭说明
Horseradish Peroxidase (HRP) -
克隆
多克隆 -
同种型
IgG -
常规说明
Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance. -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
Use at an assay dependent dilution.
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| ELISA |
1/1000.
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| IHC-P |
Use at an assay dependent dilution.
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|
| IHC-Fr |
Use at an assay dependent dilution.
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| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| ICC/IF |
1/1000 - 1/5000.
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| WB | (10) |
1/2000 - 1/10000. PubMed: 17200442
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| 说明 |
|---|
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Dot blot
Use at an assay dependent dilution. |
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ELISA
1/1000. |
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IHC-P
Use at an assay dependent dilution. |
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IHC-Fr
Use at an assay dependent dilution. |
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Immunomicroscopy
Use at an assay dependent dilution. |
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ICC/IF
1/1000 - 1/5000. |
|
WB
1/2000 - 1/10000. PubMed: 17200442 |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)Gao et al PLoS One. 2016 Apr 28;11(4):e0154419. doi: 10.1371/journal.pone.0154419. eCollection 2016. Fig2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.
Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).
Black arrows indicate double-positive cells.
Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H2O2. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.
Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) -
Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)This image is courtesy of an anonymous Abreview.ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.
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ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (677)
ab6728 被引用在 677 文献中.
- Zhou C et al. Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment. Cell Death Differ 28:715-729 (2021). PubMed: 32929219
- Song T et al. Zearalenone exposure affects the Wnt/ß-catenin signaling pathway and related genes of porcine endometrial epithelial cells in vitro. Anim Biosci 34:993-1005 (2021). PubMed: 32898953
- Qiu J et al. Role of TGF-ß1-mediated epithelial-mesenchymal transition in the pathogenesis of tympanosclerosis. Exp Ther Med 21:6 (2021). PubMed: 33235615
- Liu X et al. The AMPK-mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis. Exp Ther Med 21:21 (2021). PubMed: 33235630
- Kashkoulinejad-Kouhi T et al. Enhancement of cisplatin sensitivity in human breast cancer MCF-7 cell line through BiP and 14-3-3? co-knockdown. Oncol Rep 45:665-679 (2021). PubMed: 33416155