为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Immunoglobulins Heavy Chain IgG

兔抗小鼠IgG H&L (HRP) (ab6728)

  • Datasheet
  • SDS
Reviews (7)Q&A (31)References (381)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
  • ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

Key features and details

  • Rabbit Anti-Mouse IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB

你可能会感兴趣

封闭
Normal Rabbit Serum (ab166640)
色原
Product image
DAB Substrate Kit (ab64238)

查看更多相关产品

概述

  • 产品名称

    兔抗小鼠IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主

    Rabbit
  • 靶标种属

    Mouse
  • 经测试应用

    适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • 免疫原

    Mouse IgG whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • 共轭说明

    Horseradish Peroxidase (HRP)
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • HRP

相关产品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

Our Abpromise guarantee covers the use of ab6728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent dilution.
ELISA 1/1000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
WB 1/2000 - 1/10000. PubMed: 17200442

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)Gao et al PLoS One. 2016 Apr 28;11(4):e0154419. doi: 10.1371/journal.pone.0154419. eCollection 2016. Fig2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.

    Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).

    Black arrows indicate double-positive cells.

    Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H2O2. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.

    Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)This image is courtesy of an anonymous Abreview.

    ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.

  • ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)
    ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (381)

    发表研究结果有使用 ab6728?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6728 被引用在 381 文献中.

    • Jiang L  et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). PubMed: 31232476
    • Zhong W  et al. Knockdown of lncRNA PVT1 alleviates high glucose-induced proliferation and fibrosis in human mesangial cells by miR-23b-3p/WT1 axis. Diabetol Metab Syndr 12:33 (2020). PubMed: 32322310
    • Tian J  et al. NF-?B inhibits the occurrence of type 1 diabetes through microRNA-150-dependent PUMA degradation. Life Sci 255:117724 (2020). PubMed: 32360624
    • Yang J  et al. Circular RNA Circ100084 functions as sponge of miR-23a-5p to regulate IGF2 expression in hepatocellular carcinoma. Mol Med Rep 21:2395-2404 (2020). PubMed: 32323783
    • Torri A  et al. Viral Infection and Stress Affect Protein Levels of Dicer 2 and Argonaute 2 in Drosophila melanogaster. Front Immunol 11:362 (2020). PubMed: 32194567
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 38 Abreviews or Q&A

    EXCELLENT SECONDARY ANTIBODY for WB

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    EXCELLENT SECONDARY ANTIBODY for WB

    Mrs. Kyunga Kwak

    Verified customer

    提交于 May 07 2020

    Very sensitive and specific secondary antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    The dilution used for this secondary antibody was 1/20000 and we obtained intesnse specific bands even at very short incubation times. It works very well with phosphorylated proteins which sometimes are harder to detect (in these cases we use 1/10000 dilution or 1/5000).

    Figure legend
    Western blot image of actin bands. HCT116 cells transfected with non-targeting siRNA (lane #1) or CDK4/6 siRNA (lane #2).

    Abcam user community

    Verified customer

    提交于 Oct 02 2018

    Immunoblot detection of B-3-Tubulin

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    20 μg of micro tubular protein from SH-SY5y cells were used to detect B-3-Tubulin (Primary antibody - 1:500 dilution overnight, Secondary antibody (ab6728 - 1: 2000 dilution for 1h).

    Abcam user community

    Verified customer

    提交于 Feb 28 2018

    Excellent 2nd antibody for WB

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    A excellent 2nd Ab for WB (1:2,000 dilution) used with ab9484 (1:10000)

    Mr. Baptiste Jude

    Verified customer

    提交于 Oct 10 2016

    This is a good 2nd antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    I used this antibody, when I perform WB.
    This antibody is very sensitive.
    Dilution concentration that I used is 1/2000 in TBST(5% BSA) for 1hour.

    Abcam user community

    Verified customer

    提交于 May 30 2014

    The product is good

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    When I perform WB, that is very sensitive. making band is clearly

    Abcam user community

    Verified customer

    提交于 Jan 23 2014

    good for mouse primary antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    strong detection

    Abcam user community

    Verified customer

    提交于 Jul 11 2013

    Question

    Our customer purchased ab6728 when we had promotion last December and opened and used recently. It showed high background so the customer tried several times but failed to optimize the condition.
    So, the customer tried other brand’s(GenDEPOT) secondary antibody and finally, he got good results. I have attached file and you can see the data.
    I explained about abpromise but the customer would like to ask your opinion and recommendation about his data.
    Please check attached file.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2012

    Answer

    Thank you for your email. I am sorry to hear that customer have been experiencing problems with this antibody.

    I have read the details you have kindly provided and have following further suggestions for troubleshooting;

    - Please try 5% BSA for blocking with 2 hours incubation
    - Heat the samples for 10 minutes 100C
    - Try no primary control

    It is true that the antibody is out of guarantee period so I am sorry we cannot provide replacement products; we can only provde the troubleshooting suggestions. If however this customer is your loyal customer then you can certainly offer them incentives for keeping them on board.

    I hope this information will be helpful. SHould you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Jul 25 2012

    Question

    ecd a problem with our product, which including mouse anti His Tag (4D11) cat no. AB5000 (lot GR44936-1) and AB6728 (Lot GR15565-6) and wants to claim this item under the abpromise guarantee. Her detailed information about her condition using to detect the protein is in the attached file.

    However, I’m not sure if it’s possible to help her to optimize the reaction and get a better result. Because her comment is that primary antibody cannot bind to her target protein at all.

    It would be my appreciate if you are able to help me to solve her problem?

    Read More

    Abcam community

    Verified customer

    Asked on May 28 2012

    Answer

    Thank you for contacting us. I am sorry to hear these antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. 1) About the secondary antibody ab6728: as indicated on the datasheet, many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. However, I would be pleased to replace, if you wish, this antibody with an alternative catalog reference : ab97046, www.abcam.com/ab97046. 2) About the anti-His tag ab5000: to our knowledge this antibody has never been tested for one hour incubation only but overnight at 4ºC. Some antibodies bind better to their target at 4ºC, some other at room temperature. I think it would be worth trying an overnight incubation at 4ºC for this antibody. The blocking solution should not be problem, this antibody works fine in milk as well as in BSA. Please let me know about the results after an overnight incubation at 4ºC. If the antibody is still not working I will be pleased to offer a replacement or a credit note. Thank you for your cooperation. I look forward to hearing from you with your results using ab5000 and with details of how you would like to proceed with ab6728.

    Read More

    Abcam Scientific Support

    回复于 May 28 2012

    Question

    Dear Technical Team,

    Hope this e-mail finds you well!

    Kindly suggest the exact concentration of Rabbit polyclonal Secondary Antibody to Mouse IgG - H+L (HRP) (ab6728) since on it's datasheet the concentration is mentioned as 1 mg at 2mg/ml .

    Looking forward towards your valued response on it.

    With Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 26 2012

    Answer

    Thank you for your message.

    I am pleased to help answer your question.

    I can confirm that '1 mg at 2mg/ml' means that the vial contains 1 mg of antibody at a concentration of 2 mg.ml. The concentration is 2 mg/ml. (Therefore the vial contains 500 ul).

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Mar 26 2012

    1-10 of 38 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 4
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有