为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Immunology Immunoglobulins Heavy Chain IgG

兔抗小鼠IgG H&L (Alkaline Phosphatase) (ab6729)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (21)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Rabbit
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB

你可能会感兴趣

封闭
Normal Rabbit Serum (ab166640)
IHC
Product image
Fast Red Substrate Kit (ab64254)
色原
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)

查看更多相关产品

概述

  • 产品名称

    兔抗小鼠IgG H&L (Alkaline Phosphatase)
    参阅全部 IgG 二抗
  • 宿主

    Rabbit
  • 靶标种属

    Mouse
  • 经测试应用

    适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • 免疫原

    Mouse IgG whole molecule

  • 偶联物

    Alkaline Phosphatase

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Diluted stock solutions should be used immediately and not be frozen. Do Not Freeze. Please see notes section. Store undiluted.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.87% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.01% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • 克隆

    多克隆
  • 常规说明

    Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Mouse
    • IgG
    • Enzyme
    • Alkaline Phos

相关产品

    应用

    Our Abpromise guarantee covers the use of ab6729 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Dot blot Use at an assay dependent concentration.
    ELISA Use at an assay dependent dilution.
    IHC-P Use at an assay dependent concentration.
    IHC-Fr Use at an assay dependent concentration.
    Immunomicroscopy Use at an assay dependent concentration.
    ICC/IF 1/500 - 1/2000.
    WB Use at an assay dependent dilution.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (21)

    发表研究结果有使用 ab6729?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6729 被引用在 21 文献中.

    • Nunes C  et al. Red wine extract preserves tight junctions in intestinal epithelial cells under inflammatory conditions: implications for intestinal inflammation. Food Funct 10:1364-1374 (2019). PubMed: 30735221
    • Kuznetsov JN  et al. BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers. Sci Adv 5:eaax1738 (2019). PubMed: 31555735
    • Shan X  et al. Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-ß-induced PI3K/ATK-activated protein C system. Exp Ther Med 17:3509-3517 (2019). PubMed: 30988731
    • Sun AC  et al. High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array. IEEE J Solid-State Circuits 53:2054-2064 (2018). PubMed: 30559530
    • Jang J  et al. Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens. Sci Rep 8:7211 (2018). PubMed: 29739951
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

    BATCH NUMBER 131853 ORDER NUMBER MBPO/023 DESCRIPTION OF THE PROBLEM No signal of the relavant antigen (I have tried two different primary: see below), many non-target bands. SAMPLE Budding yeast cell extract (PCNA-Myc and non-tagged). PRIMARY ANTIBODY Abcam ab56 anti-Myc (9E11), Lot 130523, 1000x to 5000x dilution, 1 hr. or Abcam ab29 anti-PCNA, Lot 119707, 1000x to 5000x dilution, 1 hr. DETECTION METHOD CDP-star reagent from Perkin Elmer. POSITIVE AND NEGATIVE CONTROLS USED For detection of Myc-tagged protein, I included non-taggad sample as negative control. Size difference in Myc-tagged and non-tagged samples must help to identify correct band. Same blot has worked fine with HRP-Anti Myc from Santa Cruz, enabled me to compare the result. ANTIBODY STORAGE CONDITIONS In fridge (4 degree) w/o dilution. SAMPLE PREPARATION Roche Complete as protease inhibitor. Boiled soon after addition of SDS. AMOUNT OF PROTEIN LOADED up to 10 ug. ELECTROPHORESIS/GEL CONDITIONS Reducing, mainly 6% and 12%. TRANSFER AND BLOCKING CONDITIONS 5% milk in PBS-T as blocking. SECONDARY ANTIBODY ab6729 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I tried several different dilution of both primary and secondary (x1000 to x5000). At 1000x dilution, backgorund was very high. At 3000x or 5000x dilution, background was reduced, but no target band. Similar pattern of background bands. I also tried "No Primay". It gave quite similar background bands. ADDITIONAL NOTES I used ab6729 in combination with ab29 anti-PCNA and ab56 anti-Myc. Both gave essentially identical result (no target band, many background). The same blot worked fine for HRP-anti Myc from Santa Cruz. Also not tested in western, I believe that ab56 anti-Myc is OK, because I and a student have used it for ChIP and got nice results. Taken together, I conclude that an6729 doesn't specifically recognize mouse IgG. I prefer replacement to other antibody with good record, such as ab6790, rather than refunding.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2005

    Answer

    Thank you for contacting us for technical support with ab6729. I think the problem you are experiencing is due to the fact that budding yeast expresses an endogenous form of alkaline phosphate (http://www.expasy.ch/uniprot/O60109) and by using a secondary antibody conjugated to AP this creates a background signal. I would therefore recommend using a HRP conjugated secondary antibody (and ECl + detection kit) to prevent this problem. Please do not hesitate to contact us again if you still experience problems with an HRP-conjugated secondary antibody,

    Read More

    Abcam Scientific Support

    回复于 Nov 25 2005

    Question

    The lot # is: 71496 and the order number: 68495 Thanks a lot.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2005

    Answer

    I have heard back from the originator of this product and would like to pass along the following comments to you (possible resaons why the AP loss activity). a). Researcher diluted the product and stored at 4oC. THE PRODUCT HAS TO BE STORED UNDILUTED. b). The product was accidently frozen, resulting in reduced activity. c). The pipette used for sampling was contiminated, that may accelarate AP degredation. That often happens. If you touched the tips, they may have been contaminated with DNAse, proteases etc. Also AP should be stored in dark tubes. d). During storage, the liquid evaporated to the cap, causing the antibody to dry. e). The substrate was at the wrong pH, or went off, so reaction was not at optimal conditions. I just wanted to pass this onto to you, please let me know if you would like a replacement vial of ab6729.

    Read More

    Abcam Scientific Support

    回复于 Apr 01 2005

    Question

    The lot # is: 71496 and the order number: 68495 Thanks a lot.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2005

    Answer

    Thank you for your email. I have contacted the originator of this antibody in order to see if they have any suggestions or have heard of any problems with the particular lot that you have received. I will not hear back until next week and I'm also going to be away next week. I will pass their comments onto you when I do hear. In the meantime, if you would like to try a replacement vial, please let me know and I or one of my colleagues will arrange that for you. Thank you again, and have a great weekend.

    Read More

    Abcam Scientific Support

    回复于 Apr 01 2005

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM loss of activity of the alkaline phosphatase SAMPLE amyloid fibers from a-beta: used anti-abeta antibody and than used the anti mouse IgG as secondary antibody PRIMARY ANTIBODY Abcam ab 10146 dilution 1:6000 SECONDARY ANTIBODY abcam ab 6729 dilution 1:1000 DETECTION METHOD UV-absorption POSITIVE AND NEGATIVE CONTROLS USED yes SAMPLE PREPARATION coated plates with amyloid, incubated with anti abeta and than with anti mouse IgG TRANSFER AND BLOCKING CONDITIONS BSA blocking HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 20 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes WHAT STEPS HAVE YOU ALTERED? incubation times and concentrations ADDITIONAL NOTES I tried the assay many times to find the right concentration, and eventually found it. the conentrations were in the described range for these antibodies. However suddenly the turnover rate of the alkaline phophatase came to almost a stand still within two weeks of using it. Normally is took 30 - 45 minutes to get the desired response. Now it takes 15-20 hours to get a comparable response, with the same time and concentrations used before. does the alkaline phosphase decompose after a certain period of time? I kept it at 4 degrees as was said in the data sheet.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2005

    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab6729. Can you please tell me the lot number that you received (it is located on the vial) and the Abcam order number or purchase order number that was used?

    Read More

    Abcam Scientific Support

    回复于 Mar 29 2005

    Question

    >lot 52644 (My PO no: 04-12-0141) Abcam Reference No: 64641 (Invoice No: 48594) > > ab8226-200 > > >1. Please describe the problem (high background, wrong band size, more bands, no band etc). > >2. On what material are you testing the antibody in WB? >.Species? Rabbit muscle tissue >.Cell extract/ Nuclear extract? Tissue extract >.Purified protein? >.Recombinant protein? > >3. How much protein did you load? 10ug >.How did you prepare the lysate for the analysis (protease inhibitors etc)? sonicate with lysis buffer >.Did you heat the samples? yes > >4. Primary Antibody >.Specification (in which species was it raised against)? ab8226-200 >.At what dilution(s) have you tested this antibody? 1:250 >.Incubation time, wash step? 1 hr , wash - 5min, 15min, 5 min and 5 min > >5. Secondary Antibody >.Specification (in which species was it raised against)? ab 6729 >.At what dilution(s) have you tested this antibody? 1:5000 >.Incubation time, wash step? 1 hr, wash - 15min, 5 min 4 times >.Do you know whether the problems you are experiencing come from the secondary? No, other antibodies are within the specify range from supplier > >6. What detection method are you using? Perkin Elmer western Lightening chemiluminescence > >7. Background bands >.Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a "No primary" control) >.Is the blocking step sufficient? Yes (we use 0.3g skimmed milk, 5ul tween 20 in 50 ml TBS.) > > .Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps) >.At what size are the bands migrating? Could they be degradation products of your target? Probably > >8. Optimization attempts >.How many times have you tried the Western? 4 times >.Do you obtain the same results every time e.g. are background bands always in the same place? I don't get any bands with the recommended dilution on the data specification sheet I get bands only when I use 1 :250 dilution. >.What steps have you altered? primary antibody dilution I have tried 1:10000, 1:1000, 1:500 and 1:250 (only the 1:250 dilution give bands) > >9. Did you apply positive and negative controls along with the samples? Please specify. No

    Read More

    Abcam community

    Verified customer

    Asked on Feb 15 2005

    Answer

    Thank you for the details that you have provided. I'm surprised that you are unable to see a signal with ab8226 except for the faint signal detected with the dilution of 1:250. We have had some good feedback from customers who have successfully used the antibody at dilutions from 1:1000 to 1:5000. I can certainly send you a replacement vial of ab8226 free of charge. Please let me know if you are interested.

    Read More

    Abcam Scientific Support

    回复于 Feb 16 2005

    Question

    I have an enquiry which I don't know whether other of your customer faces it. I had bought ab8226 (Anti beta actin) a few weeks ago and according to the data sheet the recommended dilution for westtern blot is between 1/500-1/10000. I have tried with 1/10000 with no bands and 1/500 with super faint band after 1 hour exposure, then I tried with 1/250 and exposed for 1 hour and I get better result, the blot is attached. I am using Ab6729 (diltuon 1/5000) as secondary antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2005

    Answer

    Thanks for your email and I'm sorry to hear that you are experiencing difficulty with ab8226. To better assist you, can you please provide me with me with more details regarding your protocol? Also, please include the lot number that you received (it is located on the vial) and the Abcam order number or purchase order number that was used. Thank you, and I look forward to hearing from you. 1. Please describe the problem (high background, wrong band size, more bands, no band etc). 2. On what material are you testing the antibody in WB? •Species? •Cell extract/ Nuclear extract? •Purified protein? •Recombinant protein? 3. How much protein did you load? •How did you prepare the lysate for the analysis (protease inhibitors etc)? •Did you heat the samples? 4. Primary Antibody •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation time, wash step? 5. Secondary Antibody •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation time, wash step? •Do you know whether the problems you are experiencing come from the secondary? 6. What detection method are you using? 7. Background bands •Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control) •Is the blocking step sufficient? (We recommend blocking the membrane by adding 20 ml of blocking buffer (5% non-fat dry milk, 0.1% Tween-20 in TBS). Incubate for 2 h at room temperature or overnight at 4°C with agitation) •Are your washing steps sufficiently stringent? (Multiple short washes are more effective than fewer longer wash steps) •At what size are the bands migrating? Could they be degradation products of your target? •Please provide an image of your blot (as an e-mail attachment, a faxed image is not sufficient) 8. Optimization attempts •How many times have you tried the Western? •Do you obtain the same results every time e.g. are background bands always in the same place? •What steps have you altered? 9. Did you apply positive and negative controls along with the samples? Please specify.

    Read More

    Abcam Scientific Support

    回复于 Feb 07 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有