概述

  • 产品名称

    兔抗小鼠IgG H&L
  • 宿主

    Rabbit
  • 靶标种属

    Mouse
  • 特异性

    Recognizes mouse IgG heavy and light chains.
  • 经测试应用

    适用于: ELISA, WBmore details
  • 免疫原

    Native, mouse whole IgG.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This product was purified by affinity chromatography using mouse IgG-agarose.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab46540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000 - 1/100000.
WB 1/2000 - 1/10000.

This product is suitable for IPPT, Immunodiffusion and most immunological methods requiring lot-to-lot consistency, high titre and specificity.

文献

This product has been referenced in:

  • Correia-Melo C  et al. Rapamycin improves healthspan but not inflammaging in nf?b1-/- mice. Aging Cell 18:e12882 (2019). Read more (PubMed: 30468013) »
  • Theka I  et al. Wnt/ß-catenin signaling pathway safeguards epigenetic stability and homeostasis of mouse embryonic stem cells. Sci Rep 9:948 (2019). Read more (PubMed: 30700782) »
See all 214 Publications for this product

客户评价及客户问答

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you for contacting us.


First, I'd like to point out some resources on our website which may help:


https://www.abcam.com/index.html?rid=310


A Beginner's Guide to ChIP - Abcam


As we discussed over the phone, you may want to start with our ChIP kit (ab500)since this has everything you need for the reaction, including the positive control antibody ab1791.


https://www.abcam.com/ChIP-Kit-ab500.html


In addition, you'll need the two antibodies you were interested in, and their matching negative controls.


Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)


https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-ChIP-Grade-ab8580.html


Rabbit Control IgG - ChIP Grade (ab46540)


https://www.abcam.com/Rabbit-Control-IgG-ChIP-Grade-ab46540.html


Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)


https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html


Mouse IgG3, kappa monoclonal [MG3-35] - Isotype control (ab18394). This one isn't tested in ChIP, but I don't see a reason why it wouldn't work.


https://www.abcam.com/Mouse-IgG3-kappa-monoclonal-MG3-35-Isotype-control-ab18394.html


You'll also need the housekeeping primers, like GAPDH, ab83588 and ab83589.

https://www.abcam.com/hsgapdh-chip-primer-exon-1-forward-ab83588.html

https://www.abcam.com/hsgapdh-chip-primer-exon-1-reverse-ab83589.html


That should be enough to get you started, but take a look at the resources mentioned above and let me know if you have any additional questions.

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Question
Answer

Merci beaucoup pour votre appel de ce matin.

Je suis désolée de devoir vous confirmer que nous devons corriger et informer les clients du produit ab46540, car, grâce à vous, j'ai vérifié et j'ai trouvéquele ab46540 est effectivement réactif contre les immunoglobulines de souris. Nous allons corriger ceci. Merci d'avoir attiré notre attention à ce fait.

Même si vous travaillez avec du tissu humain, je ne préfère pas vous recommander le ab46540. Le ab37415 est aussi un control pour les IgG de lapin et il était aussi testé en ChIP. Je peux donc vous recommander celui- ci. J'ai aussi double vérifié que le ab37415 ne contient effectivement aucune réactivité connue.

J'espère que ces informations vous sont utiles. Je m'excuse pour ces confusions et nous restons à votre disposition pour tout renseignement supplémentaire.

Read More

Answer

Thank you for your inquiry.

Indeed we do have also polyclonal control fractions of mouse or rat IgGs.

For the mouse IgG, we do have the IgG fraction ab37355 https://www.abcam.com/index.html?datasheet=37355 (or use the following: https://www.abcam.com/index.html?datasheet=37355).

For the rat IgG, we do have the IgG fraction ab37361 https://www.abcam.com/index.html?datasheet=37361 (or use the following: https://www.abcam.com/index.html?datasheet=37361).

These two products have been used as controls so far only in ELISA and flow cytometry to our knowledge. As they have however no known specificity, I would expect them to work also in ChIP. If you would like to test them in ChIP, please do let me know, as I might can provide you with a testing offer.

Otherwise, if you are looking for a monoclonal rat or mouse antibody as a control, we do have also monoclonal isotype controls. Voici la liste de nos anticorps isotypes controls: https://www.abcam.com/index.html?pageconfig=catalog_byclass&intClassID=718&strStartChar=A,B,C,D,E,F,G,H,I,J,K,L,M

Please do not hesitate to contact us again if you have anyquestion, if wecould help you in the further selection of an antibody or if you are interested in testing the above mentioned antibodies in ChIP.

Read More

Answer

Thank you for your enquiry.

I have confirmed the purification details of the antibodies you listed and have updated the datasheets where necessary:

ab3609 (Aurora B)
Protein G purified

ab51841 (TATA binding protein)
Protein G purified

ab37373 (goat control IgG)
Proprietary combination of ammonium sulfate precipitation, ion exchange, and/or other chromatography techniques.

ab46540 (rabbit control IgG)
Affinity purified against the antigen.

I hope this will be helpful. If you require any further information on the purification of other products, or any other questions, please do not hesitate to contact me.

Read More

Answer

Thank you for your inquiry.
I can confim that the antibody was affinity-purified against the antigen itself (bound to agarose).
Protein A or G was not used.
I hope this information is helpful. please do not hesitate to contact me with any further questions.

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