重组兔单克隆抗体[H19-5]抗人kappa light chain (ab125919)
Key features and details
- Rabbit monoclonal [H19-5] Anti-Human kappa light chain
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA
Related conjugates and formulations
概述
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产品名称
兔单克隆抗体[H19-5]抗人kappa light chain
参阅全部 Kappa light chain 二抗 -
宿主
Rabbit -
靶标种属
Human -
经测试应用
适用于: WB, ELISAmore details -
免疫原
Other Immunogen Type corresponding to Human Kappa light chain.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
H19-5 -
同种型
IgG -
常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125919于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000.
For unpurified use at 1/1000 - 1/10000. |
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ELISA |
Use a concentration of 1 µg/ml.
For unpurified use at 1/1000 - 1/10000. |
说明 |
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WB
1/5000. For unpurified use at 1/1000 - 1/10000. |
ELISA
Use a concentration of 1 µg/ml. For unpurified use at 1/1000 - 1/10000. |
图片
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All lanes : Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution
Lane 1 : Human spleen tissue lysate
Lane 2 : Human thymus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution (purified) + Human tonsil tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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All lanes : Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) (unpurified)
Lane 1 : Human spleen tissue lysate
Lane 2 : Human thymus tissue lysate
Lane 3 : Human tonsil tissue lysate -
ELISA dose-responsive curve using purified ab125919 at a range of concentrations.
Antigen concentation: 1000ng/ml.
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab125919 被引用在 3 文献中.
- Hunt AC et al. A rapid cell-free expression and screening platform for antibody discovery. Nat Commun 14:3897 (2023). PubMed: 37400446
- Zhang H et al. microRNA-4701-5p protects against interleukin-1β induced human chondrocyte CHON-001 cells injury via modulating HMGA1. J Orthop Surg Res 17:246 (2022). PubMed: 35459188
- Seo Y et al. Assembly and Folding Properties of Cytosolic IgG Intrabodies. Sci Rep 10:2140 (2020). PubMed: 32034177