Application
Western blot
Experimental conditions:
MCF-7 and MDA-MB-231 whole cell lysates extracted with RIPA buffer.
10% SDS-PAGE, 50,000 cells loaded into wells.
Blocking 1h with 5% milk in TBS at RT.
Incubation with primary anti-Vimentin (ab92547) 1:5000 overnight at 4ºC.
Incubation with secondary antibody 0.05ug/ml for 1h at RT.
Developed with ECL for 5 minutes.
Results:
No nonspecific binding. No background. Clear bands.
MCF-7 and MDA-MB-231 whole cell lysates extracted with RIPA buffer.
10% SDS-PAGE, 50,000 cells loaded into wells.
Blocking 1h with 5% milk in TBS at RT.
Incubation with primary anti-Vimentin (ab92547) 1:5000 overnight at 4ºC.
Incubation with secondary antibody 0.05ug/ml for 1h at RT.
Developed with ECL for 5 minutes.
Results:
No nonspecific binding. No background. Clear bands.
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提交于 Nov 19 2014