兔抗人IgG H&L (HRP) (ab6759)
Key features and details
- Rabbit Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA
Related conjugates and formulations
概述
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产品名称
兔抗人IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Human -
经测试应用
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details -
免疫原
human IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Horseradish Peroxidase (HRP) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
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Dot blot |
Use at an assay dependent dilution.
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ELISA | (1) |
1/130000.
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IHC-P |
Use at an assay dependent dilution.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
WB |
1/5000 - 1/25000.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
Competitive ELISA |
Use at an assay dependent dilution. PubMed: 20573830
|
说明 |
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Dot blot
Use at an assay dependent dilution. |
ELISA
1/130000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/5000 - 1/25000. |
ICC/IF
1/1000 - 1/5000. |
Competitive ELISA
Use at an assay dependent dilution. PubMed: 20573830 |
图片
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ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (115)
ab6759 被引用在 115 文献中.
- Zhang X et al. Altered genome‑wide hydroxymethylation analysis for neoadjuvant chemoradiotherapy followed by surgery in esophageal cancer. Exp Ther Med 25:29 (2023). PubMed: 36561617
- Umemoto A et al. Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther 25:25 (2023). PubMed: 36804906
- Zeng Q et al. TRIM59 attenuates ox-LDL-induced endothelial cell inflammation, apoptosis, and monocyte adhesion through AnxA2. Ann Transl Med 11:42 (2023). PubMed: 36819529
- Martin-Souto L et al. Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients. J Fungi (Basel) 9:N/A (2023). PubMed: 36836272
- Yang J et al. KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma. Exp Ther Med 25:154 (2023). PubMed: 36911368