兔抗人IgG H&L (HRP) (ab6759)

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ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

    Key features and details

    • Rabbit Anti-Human IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Rabbit
    • Isotype: IgG
    • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA

    概述

    • 产品名称

      兔抗人IgG H&L (HRP)
      参阅全部 IgG 二抗

    • 宿主

      Rabbit

    • 靶标种属

      Human

    • 经测试应用

      适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details

    • 免疫原

      human IgG whole molecule

    • 偶联物

      HRP

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 纯度

      Affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 共轭说明

      Horseradish Peroxidase (HRP)

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    相关产品

      应用

      The Abpromise guarantee

      Abpromise™承诺保证使用ab6759于以下的经测试应用

      “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

      应用 Ab评论 说明
      Dot blot
      Use at an assay dependent dilution.
      ELISA (1)
      1/130000.
      IHC-P
      Use at an assay dependent dilution.
      IHC-Fr
      Use at an assay dependent dilution.
      Immunomicroscopy
      Use at an assay dependent dilution.
      WB
      1/5000 - 1/25000.
      ICC/IF
      1/1000 - 1/5000.
      Competitive ELISA
      Use at an assay dependent dilution. PubMed: 20573830
      说明
      Dot blot
      Use at an assay dependent dilution.
      ELISA
      1/130000.
      IHC-P
      Use at an assay dependent dilution.
      IHC-Fr
      Use at an assay dependent dilution.
      Immunomicroscopy
      Use at an assay dependent dilution.
      WB
      1/5000 - 1/25000.
      ICC/IF
      1/1000 - 1/5000.
      Competitive ELISA
      Use at an assay dependent dilution. PubMed: 20573830

      图片

      • ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
        ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)

        ELISA results of ab6759 tested against purified human IgG protein.

        Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (49)

      发表研究结果有使用 ab6759？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6759 被引用在 49 文献中.

      • Zhu LJ  et al. BUB1 promotes proliferation of liver cancer cells by activating SMAD2 phosphorylation. Oncol Lett 19:3506-3512 (2020). PubMed: 32269624
      • Orlowski A  et al. Successful Transduction with AAV Vectors after Selective Depletion of Anti-AAV Antibodies by Immunoadsorption. Mol Ther Methods Clin Dev 16:192-203 (2020). PubMed: 32055647
      • Yuan YF  et al. Cyclopamine functions as a suppressor of benign prostatic hyperplasia by inhibiting epithelial and stromal cell proliferation via suppression of the Hedgehog signaling pathway. Int J Mol Med 46:311-319 (2020). PubMed: 32319534
      • Wang X  et al. Adenovirus delivery of encoded monoclonal antibody protects against different types of influenza virus infection. NPJ Vaccines 5:57 (2020). PubMed: 32665862
      • Eick GN  et al. Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots. J Physiol Anthropol 39:16 (2020). PubMed: 32678000
      View all Publications for this product

      客户评价及客户问答

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      Intracellular Alpha-galactosidase A/antibody-complex detection from enzyme-treated EA.hy926 cells

       Excellent
      Abreviews
      abreview image
      Application
      ELISA
      Cells were seeded on 96-well plates with a density of 2x105 cell/ml and grown until confluent. After 4 hours of incubation with human recombinant α-galactosidase A (AGAL, 4.6 μg/ml) and patients’ serum (50 µl per 4.6 μg/ml AGAL), cells were washed twice with PBS and lysed with passive lysis buffer (Promega, Mannheim Germany). Lysates were centrifuged (14.000x g) for 20 minutes at 4°C and supernatants were used for ELISAs, as follows. 96 well plates were coated with a capture antibody (α-AGAL, R&D Sytems, Minneapolis, US no. AF6146, 200 ng per well) over night at 4°C. After 5 washes with 200 μl PBS/Tween 0.1% and blocking with PBS/BSA 2% for 2 hours at RT, 3-10 μl of cellular lysates were applied in 100 μl PBS overnight at 4°C. Wells were washed 3 times with PBS/Tween 0.1%. For human IgG detection shuttled into cells by AGAL, anti-hIgG HRP-coupled antibodies (Abcam no: ab6759, working concentration: 0.66 μg/ml) were applied in PBS/BSA 2% for 2 hours at RT. After 5 additional washes, 100 μl 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added to each well, incubated at RT, and stopped with 100 μl 2 mol/l sulfuric acid. Absorption was measured at 450 nm and normalized for protein content and against serum from a healthy control (negativ for antibodies against AGAL).

      Figure legend: *p<0.05, **p<0.01.

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      提交于 Sep 04 2019

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