兔抗人IgG H&L (HRP) (ab6759)
Key features and details
- Rabbit Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISA
Related conjugates and formulations
概述
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产品名称
兔抗人IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Human -
经测试应用
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details -
免疫原
human IgG whole molecule
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Horseradish Peroxidase (HRP) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
Dot blot |
Use at an assay dependent dilution.
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ELISA | (1) |
1/130000.
|
IHC-P |
Use at an assay dependent dilution.
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|
IHC-Fr |
Use at an assay dependent dilution.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
WB |
1/5000 - 1/25000.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
Competitive ELISA |
Use at an assay dependent dilution. PubMed: 20573830
|
说明 |
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Dot blot
Use at an assay dependent dilution. |
ELISA
1/130000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/5000 - 1/25000. |
ICC/IF
1/1000 - 1/5000. |
Competitive ELISA
Use at an assay dependent dilution. PubMed: 20573830 |
图片
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ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (65)
ab6759 被引用在 65 文献中.
- Bühler L et al. Lipocalin 13 enhances insulin secretion but is dispensable for systemic metabolic control. Life Sci Alliance 4:N/A (2021). PubMed: 33536239
- Sotoudegan F et al. Anti-Aß-scFv-loaded polymeric nano-micelles with enhanced plasma stability. J Pharm Pharmacol 73:460-472 (2021). PubMed: 33793837
- Yusupov M et al. Oxidative damage to hyaluronan-CD44 interactions as an underlying mechanism of action of oxidative stress-inducing cancer therapy. Redox Biol 43:101968 (2021). PubMed: 33895486
- Iizasa E et al. TREM2 is a receptor for non-glycosylated mycolic acids of mycobacteria that limits anti-mycobacterial macrophage activation. Nat Commun 12:2299 (2021). PubMed: 33863908
- Fan J et al. Oncolytic herpes simplex virus and temozolomide synergistically inhibit breast cancer cell tumorigenesis in vitro and in vivo. Oncol Lett 21:99 (2021). PubMed: 33376532