兔抗人IgG H&L (Alkaline Phosphatase) (ab6760)

Submit a review Q&A (1)References (4)

Key features and details

  • Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISA

概述

  • 产品名称

    兔抗人IgG H&L (Alkaline Phosphatase)
    参阅全部 IgG 二抗

  • 宿主

    Rabbit

  • 靶标种属

    Human

  • 经测试应用

    适用于: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISAmore details

  • 免疫原

    human IgG whole molecule

  • 偶联物

    Alkaline Phosphatase

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 50% Glycerol
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.

  • 共轭说明

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • 克隆

    多克隆

  • 研究领域

相关产品

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6760于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Immunomicroscopy
    WB
    ICC/IF
    IHC-Fr
    Dot blot
    IHC-P
    ELISA
    说明
  • 应用说明
    suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency

    • 实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (4)

    发表研究结果有使用 ab6760？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6760 被引用在 4 文献中.

    • Royle KE & Polizzi K A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Sci Rep 7:15817 (2017). WB . PubMed: 29150665
    • Iversen R  et al. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91 (2013). PubMed: 23690478
    • Kap YS  et al. Fast Progression of Recombinant Human Myelin/Oligodendrocyte Glycoprotein (MOG)-Induced Experimental Autoimmune Encephalomyelitis in Marmosets Is Associated with the Activation of MOG34-56-Specific Cytotoxic T Cells. J Immunol 180:1326-37 (2008). ELISA . PubMed: 18209026
    • Jagessar SA  et al. Autoimmunity Against Myelin Oligodendrocyte Glycoprotein Is Dispensable for the Initiation Although Essential for the Progression of Chronic Encephalomyelitis in Common Marmosets. J Neuropathol Exp Neurol 67:326-340 (2008). ELISA . PubMed: 18379435

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Question

    does this antibody recognize human kappa light chains? lambda? both?

    Read More
    Answer

    ab6760 is produced against polyclonal whole human IgG purified from serum, and the kappa:lambda ratio of IgG normal human serum is reported to be ~2:1. ab6760 is not tested specifically against kappa or lambda light chains, but it is expected to recognize both forms as they are normally present in human serum.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com