兔抗人IgG H&L (Alkaline Phosphatase) (ab6760)

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Key features and details

  • Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISA

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概述

  • 产品名称

    兔抗人IgG H&L (Alkaline Phosphatase)
    参阅全部 IgG 二抗

  • 宿主

    Rabbit

  • 靶标种属

    Human

  • 经测试应用

    适用于: Immunomicroscopy, WB, ICC/IF, IHC-Fr, Dot blot, IHC-P, ELISAmore details

  • 免疫原

    human IgG whole molecule

  • 偶联物

    Alkaline Phosphatase

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 50% Glycerol
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.

  • 共轭说明

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6760于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Immunomicroscopy
WB
ICC/IF
IHC-Fr
Dot blot
IHC-P
ELISA
说明
应用说明
suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4)

发表研究结果有使用 ab6760？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6760 被引用在 4 文献中.

  • Royle KE & Polizzi K A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Sci Rep 7:15817 (2017). WB . PubMed: 29150665
  • Iversen R  et al. Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells. J Immunol 190:5981-91 (2013). PubMed: 23690478
  • Kap YS  et al. Fast Progression of Recombinant Human Myelin/Oligodendrocyte Glycoprotein (MOG)-Induced Experimental Autoimmune Encephalomyelitis in Marmosets Is Associated with the Activation of MOG34-56-Specific Cytotoxic T Cells. J Immunol 180:1326-37 (2008). ELISA . PubMed: 18209026
  • Jagessar SA  et al. Autoimmunity Against Myelin Oligodendrocyte Glycoprotein Is Dispensable for the Initiation Although Essential for the Progression of Chronic Encephalomyelitis in Common Marmosets. J Neuropathol Exp Neurol 67:326-340 (2008). ELISA . PubMed: 18379435

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Question

does this antibody recognize human kappa light chains? lambda? both?

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Answer

ab6760 is produced against polyclonal whole human IgG purified from serum, and the kappa:lambda ratio of IgG normal human serum is reported to be ~2:1. ab6760 is not tested specifically against kappa or lambda light chains, but it is expected to recognize both forms as they are normally present in human serum.

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