Application
Immunohistochemistry (PFA fixed)
Tissue was treated with EDTA (antigen retrieval) plus H202/serum blocking.
1st antibody was incubated with goat anti CD68: 1/200, 30 min at 21°C.
2nd HRP-conjugated secondary (ab6741): 1/1000, 1 h at 21°C.
Counterstaining with haematoxylin.
Staining was very specific.
1st antibody was incubated with goat anti CD68: 1/200, 30 min at 21°C.
2nd HRP-conjugated secondary (ab6741): 1/1000, 1 h at 21°C.
Counterstaining with haematoxylin.
Staining was very specific.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 09 2021