兔F(ab')2抗大鼠IgG - H&L (FITC),预吸附二抗(ab6246)
Key features and details
- Rabbit F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
概述
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产品名称
兔F(ab')2抗大鼠IgG - H&L (FITC),预吸附二抗
参阅全部 IgG 二抗 -
描述
兔F(ab')2抗大鼠IgG - H&L (FITC),预吸附二抗 -
宿主
Rabbit -
靶标种属
Rat -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. -
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details -
最小交叉反应
Cow, Horse, Human, Mouse, Rabbit, Sheep more details -
免疫原
Rat IgG whole molecule
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Immunomicroscopy | ||
| Flow Cyt | ||
| ELISA |
| 说明 |
|---|
应用说明
Flow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab6246 被引用在 1 文献中.
- Das R et al. Transforming Growth Factor ß1-induced Apoptosis in Podocytes via the Extracellular Signal-regulated Kinase-Mammalian Target of Rapamycin Complex 1-NADPH Oxidase 4 Axis. J Biol Chem 290:30830-42 (2015). PubMed: 26565025
