Key features and details
- Rabbit F(ab')2 Anti-Rat IgG - H&L (FITC), pre-adsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
产品名称兔F(ab')2抗大鼠IgG - H&L (FITC),预吸附二抗
参阅全部 IgG 二抗
描述兔F(ab')2抗大鼠IgG - H&L (FITC),预吸附二抗
特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
经测试应用适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
Cow, Horse, Human, Mouse, Rabbit, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rat IgG whole molecule
偶联物FITC. Ex: 493nm, Em: 528nm
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
共轭说明Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Goat IgG F(ab’)2
Our Abpromise guarantee covers the use of ab6246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6246 被引用在 1 文献中.
- Das R et al. Transforming Growth Factor ß1-induced Apoptosis in Podocytes via the Extracellular Signal-regulated Kinase-Mammalian Target of Rapamycin Complex 1-NADPH Oxidase 4 Axis. J Biol Chem 290:30830-42 (2015). PubMed: 26565025