兔抗鸡IgY H&L (HRP) (ab97140)
Key features and details
- Rabbit Anti-Chicken IgY H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
概述
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产品名称
兔抗鸡IgY H&L (HRP)
参阅全部 IgY 二抗 -
宿主
Rabbit -
靶标种属
Chicken -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
经测试应用
适用于: ICC, IHC-P, ELISA, WBmore details -
偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
相关产品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97140于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
|
|
| IHC-P |
1/200 - 1/5000.
|
|
| ELISA |
1/10000 - 1/100000. (Primary).
|
|
| WB |
1/2000 - 1/20000. Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000
|
| 说明 |
|---|
|
ICC
Use at an assay dependent dilution. |
|
IHC-P
1/200 - 1/5000. |
|
ELISA
1/10000 - 1/100000. (Primary). |
|
WB
1/2000 - 1/20000. Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000 |
图片
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Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab97140)All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab97140 被引用在 6 文献中.
- Hu R et al. An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in Poultry. Nanomaterials (Basel) 10:N/A (2020). PubMed: 33233490
- Nery-Flores SD et al. Dietary Curcumin Prevented Astrocytosis, Microgliosis, and Apoptosis Caused by Acute and Chronic Exposure to Ozone. Molecules 24:N/A (2019). PubMed: 31387223
- Wolfes AC et al. A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes. J Gen Physiol 149:149-170 (2017). PubMed: 27908976
- Nakao LS et al. Mechanism-based proteomic screening identifies targets of thioredoxin-like proteins. J Biol Chem 290:5685-95 (2015). PubMed: 25561728
- Thiede-Stan NK et al. Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex. J Cell Sci 128:3583-96 (2015). PubMed: 26290381
- Rainczuk A et al. Evidence for the antagonistic form of CXC-motif chemokine CXCL10 in serous epithelial ovarian tumours. Int J Cancer 134:530-41 (2014). PubMed: 23873303