兔抗鸡IgY H&L (FITC) (ab6749)

Submit a review Q&A (2)References (9)

Key features and details

  • Rabbit Anti-Chicken IgY H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase Biotin DyLight® 488 DyLight® 594 Gold 10nm Gold 15nm HRP Texas Red ® TRITC Unconjugated

概述

  • 产品名称

    兔抗鸡IgY H&L (FITC)
    参阅全部 IgY 二抗

  • 宿主

    Rabbit

  • 靶标种属

    Chicken

  • 经测试应用

    适用于: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details

  • 免疫原

    chicken IgY whole molecule

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6749于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000. PubMed: 18983843
ELISA
1/10000 - 1/50000.
说明
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000. PubMed: 18983843
ELISA
1/10000 - 1/50000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (9)

发表研究结果有使用 ab6749？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6749 被引用在 9 文献中.

  • Kiese K  et al. Epigenetic control of epilepsy target genes contributes to a cellular memory of epileptogenesis in cultured rat hippocampal neurons. Acta Neuropathol Commun 5:79 (2017). PubMed: 29089052
  • Cheng YC  et al. Albumin overload down-regulates integrin-ß1 through reactive oxygen species-endoplasmic reticulum stress pathway in podocytes. J Biochem 158:101-8 (2015). PubMed: 25713411
  • Buijs PR  et al. Different responses of human pancreatic adenocarcinoma cell lines to oncolytic Newcastle disease virus infection. Cancer Gene Ther 21:24-30 (2014). PubMed: 24384773
  • Na GH  et al. Effects of glucose concentration in the medium on rat hepatocyte culture. Ann Surg Treat Res 87:53-60 (2014). ICC/IF . PubMed: 25114883
  • Boulanger CA  et al. Embryonic stem cells are redirected to non-tumorigenic epithelial cell fate by interaction with the mammary microenvironment. PLoS One 8:e62019 (2013). IHC-P ; Mouse . PubMed: 23637952
  • Higgins S  et al. Inducing neurite outgrowth by mechanical cell stretch. Biores Open Access 2:212-6 (2013). ICC/IF . PubMed: 23741633
  • Kuo YC  et al. Capillary electrophoresis of bone marrow stromal cells with uptake of heparin-functionalized poly(lactide-co-glycolide) nanoparticles during differentiation towards neurons. Electrophoresis 31:315-23 (2010). ICC/IF . PubMed: 20084630
  • Tomasi ML  et al. S-adenosylmethionine regulates apurinic/apyrimidinic endonuclease 1 stability: implication in hepatocarcinogenesis. Gastroenterology 136:1025-36 (2009). ICC/IF . PubMed: 18983843
  • Papalouka V  et al. Muscle LIM protein interacts with cofilin 2 and regulates F-actin dynamics in cardiac and skeletal muscle. Mol Cell Biol 29:6046-58 (2009). ICC/IF . PubMed: 19752190

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1-2 of 2 Abreviews or Q&A

Question

I want to label the antiserum of chicken, which product I should chose for my Flowcytomry analysis (specifically FITC) among ab6749,ab7114 and ab6873? I noticed that ab7114's immunogen is full length native chicken IgY (purified). In addition, whether these there products can be replaced for each other?

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Answer

Thank you for your enquiry. ab6749, ab7114 and ab6873 are all suitable for flow cytometry analysis, have all been raised against chicken IgY and are all FITC conjugated. There is very little difference between the three aside from the mass of antibody sold. ab6749 is sold as 1.5mg, whereas ab7114 is sold as 100 µg and ab6873 as 1 mg. All are sold at the same price £ 98.00/$ 150.00/ € 165.00.

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Question

What protocols have been used for this antibody in immunofluorescence? Blocking, incubation times, dilutions. Any specific reference?

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Answer

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. We do not have a specific protocol for this antibody in immunofluorescence, or a specific reference. If you have any more questions, please contact us again.

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