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Secondary antibodies anti-Chicken IgY Biotin

兔抗鸡IgY H&L (Biotin) (ab6752)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (9)

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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)

Key features and details

  • Rabbit Anti-Chicken IgY H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Rabbit
  • Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IF

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    兔抗鸡IgY H&L (Biotin)
    参阅全部 IgY 二抗
  • 宿主

    Rabbit
  • 靶标种属

    Chicken
  • 经测试应用

    适用于: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details
  • 免疫原

    chicken IgY whole molecule

  • 偶联物

    Biotin

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • 共轭说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • 克隆

    多克隆
  • 研究领域

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Biotin

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6752于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/2000 - 1/20000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/500000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
说明
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/2000 - 1/20000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/500000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.

图片

  • Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Ab6752 was used with the primary antibody ab13989 in ICC/IF. See Abreview on ab13989.
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Ab6752 was used at dilution 1/1000 with the primary antibody ab13970 in IHC-Fr. See the review on ab13970.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (9)

发表研究结果有使用 ab6752?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6752 被引用在 9 文献中.

  • López-Fernández A  et al. Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model. Tissue Eng Part A 26:993-1004 (2020). PubMed: 32122263
  • McKenzie JA  et al. Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing. FASEB J 31:882-892 (2017). PubMed: 27864378
  • Ren J  et al. Imaging rhodopsin degeneration in vivo in a new model of ocular ischemia in living mice. FASEB J 30:612-23 (2016). PubMed: 26443823
  • Weis B  et al. Inhibition of intestinal tumor formation by deletion of the DNA methyltransferase 3a. Oncogene 0:N/A (2014). PubMed: 24837369
  • Jiao TT  et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P . PubMed: 24294399
  • Gakis G  et al. XPA-210: a new proliferation marker to characterize tumor biology and progression of renal cell carcinoma. World J Urol 29:801-6 (2011). IHC-P . PubMed: 21113600
  • Wong EW  et al. Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event. Proc Natl Acad Sci U S A 107:11399-404 (2010). IHC-Fr . PubMed: 20534521
  • Koryakina YA  et al. Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. Respir Res 9:32 (2008). ICC/IF . PubMed: 18423037
  • Ergün S  et al. Cell type-specific expression of LINE-1 open reading frames 1 and 2 in fetal and adult human tissues. J Biol Chem 279:27753-63 (2004). PubMed: 15056671

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Do you know if it would be possible to add a %glycerol to this product and keep under -20 storage conditions unaliquoted? Thank you.

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Abcam community

Verified customer

Asked on Aug 04 2006

Answer

Thank you for your enquiry. Antibodies can be stored with glyserol at -20oC. However, there is some evidence that this can sometimes decrease the activity of the antibody which means you may have to use the antibody at a higher concentration in your experiments. I hope this helps. Please get back to me if you have any more questions, and I will be happy to help. With thanks.

Read More

Abcam Scientific Support

回复于 Aug 08 2006

Question

What about working dilutions of this antibody?

Read More

Abcam community

Verified customer

Asked on Jun 28 2001

Answer

We recommend the following dilutions: ELISA 1:5,000 - 1:20,000 Immunoblotting 1:3,000 - 1:10,000 Immunohistochemistry 1:100 - 1:1,000

Read More

Abcam Scientific Support

回复于 Jun 28 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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