Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Ab5745 staining Armenian hamster IgG by Immunohistochemistry (IHC-P) using the spleen of a healthy C57BL/6J mouse. Tissue was fixed with formalin. Antigen retrieval was by heat mediation in citrate buffer, carried out in a pressure cooker. Tissue sections were blocked with 2.5% goat serum for 2 hours at room temperature, followed by incubation with primary antibody (ab231545 1/100 in 1% BSA) overnight at 4°C. The secondary antibody (1/300 in 1% BSA) was added for 40 min at room temperature.
(The image shows the second antibody control stain for positive stain please see ab231545)
(The image shows the second antibody control stain for positive stain please see ab231545)
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提交于 Sep 04 2018