Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
100000 cells
Specification
Hela
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 %
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: GE Biosciences NA931V
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: 55, 60 kDa
Positive control
Hela cells total cell lysate and purified endosomes.
Negative control
Nuclear fraction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Maria C Johansson
Verified customer
提交于 Feb 19 2007