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使用敲除细胞株进行验证重组RabMAb

重组Anti-RAB7抗体[EPR7589] (ab137029)

  • Datasheet
  • SDS
Reviews (23) Submit a question References (69)

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Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Flow Cytometry (Intracellular) - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Flow Cytometry (Intracellular) - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)
  • Anti-RAB7 antibody [EPR7589] (ab137029)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7589] to RAB7
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC Carrier Free HRP PE

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概述

  • 产品名称

    Anti-RAB7抗体[EPR7589]
    参阅全部 RAB7 一抗
  • 描述

    兔单克隆抗体[EPR7589] to RAB7
  • 宿主

    Rabbit
  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Wild-type HAP1 cell lysate; HeLa, A375, A431, U87-MG, HT1080, L929, NIH/3T3, Raw 264.7 and C2C12 cell lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa, NIH/3T3 and HepaRG cells. Flow Cyt (intra): HAP1-WT cells; A431 cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR7589
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

相关产品

  • Alternative Versions

    • Alexa Fluor® 647 Anti-RAB7 antibody [EPR7589] (ab198337)
    • Anti-RAB7 antibody [EPR7589] - BSA and Azide free (ab214806)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human RAB7A (RAB7) knockout HeLa cell line (ab255423)
  • KO cell lysates

    • Human RAB7A (RAB7) knockout HeLa cell lysate (ab263831)
  • Positive Controls

    • A-431 membrane extract lysate (ab30134)
    • NIH/3T3 whole cell lysate (ab7179)
    • C2C12 whole cell lysate (ab7182)
    • RAW 264.7 whole cell lysate (ab7187)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human RAB7 protein (ab103507)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab137029于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (12)
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (8)
1/100 - 1/500.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/500.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.
  • 组织特异性

    Widely expressed; high expression found in skeletal muscle.
  • 疾病相关

    Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.
  • 序列相似性

    Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位

    Late endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
  • Target information above from: UniProt accession P51149 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7879 Human
    • Entrez Gene: 19349 Mouse
    • Entrez Gene: 29448 Rat
    • Omim: 602298 Human
    • SwissProt: P51149 Human
    • SwissProt: P51150 Mouse
    • SwissProt: P09527 Rat
    • Unigene: 741172 Human
    • Unigene: 333233 Mouse
    • Unigene: 486309 Mouse
    • Unigene: 1425 Rat
    see all
  • 别名

    • CMT2B antibody
    • PRO2706 antibody
    • PSN antibody
    • RAB7, member RAS oncogene family antibody
    • RAB7A antibody
    • RAB7A, member RAS oncogene family antibody
    • RAB7A_HUMAN antibody
    • Ras associated protein RAB7 antibody
    • Ras related protein Rab7 antibody
    • Ras related protein Rab7a antibody
    • Ras-related protein Rab-7a antibody
    see all

图片

  • Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
    Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
    All lanes : Anti-RAB7 antibody [EPR7589] (ab137029) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RAB7A knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab137029 observed at 23 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab137029 was shown to react with RAB7 in wild-type HeLa cells in Western blot with loss of signal observed in RAB7A knockout cell line ab255423 (RAB7A knockout cell lysate ab263831). Wild-type HeLa and RAB7A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab137029 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labelling RAB7 with ab137029 at 1:250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1:1000 dilution (Green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1:200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1:1000 dilution.

  • Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
    Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
    All lanes : Anti-RAB7 antibody [EPR7589] (ab137029) at 1/1000 dilution

    Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysates
    Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
    Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RAB7 knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: Human fetal brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab137029 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab137029 was shown to specifically react with RAB7 in wild-type HAP1 cells. No band was observed when RAB7 knockout samples were examined. Wild-type and RAB7 knockout samples were subjected to SDS-PAGE. ab137029 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) labelling RAB7 with purified ab137029 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-RAB7 antibody [EPR7589] (ab137029)
    Flow Cytometry (Intracellular) - Anti-RAB7 antibody [EPR7589] (ab137029)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-RAB7 knockout cells (red line) stained with ab137029. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab137029, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 22°C. A rabbit IgG1 isotype control antibody (ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-RAB7 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min) permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody [EPR7589] (ab137029)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody [EPR7589] (ab137029)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RAB7 with ab137029 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
    Western blot - Anti-RAB7 antibody [EPR7589] (ab137029)
    All lanes : Anti-RAB7 antibody [EPR7589] (ab137029) at 1/1000 dilution

    Lane 1 : A375 (human malignant melanoma cell line) cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 3 : U87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : HT 1080 (human fibrosarcoma cell line) cell lysate
    Lane 5 : L929 (mouse connective tissue fibroblast cell line) cell lysate
    Lane 6 : NIH 3T3 (mouse embyro fibroblast cell line) cell lysate
    Lane 7 : Raw 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 8 : C2C12 (mouse myoblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 23 kDa

  • Flow Cytometry (Intracellular) - Anti-RAB7 antibody [EPR7589] (ab137029)
    Flow Cytometry (Intracellular) - Anti-RAB7 antibody [EPR7589] (ab137029)

    Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma cell line) cells labeling RAB7 with purified ab137029 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] (ab137029)This image is courtesy of an Abreview submitted by Alina Macovei

    ab137029 staining RAB7 in Human HepaRG cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in 1% milk) for 30 minutes. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Anti-RAB7 antibody [EPR7589] (ab137029)
    Anti-RAB7 antibody [EPR7589] (ab137029)

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (69)

发表研究结果有使用 ab137029?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab137029 被引用在 69 文献中.

  • Wei D  et al. RAB31 marks and controls an ESCRT-independent exosome pathway. Cell Res 31:157-177 (2021). PubMed: 32958903
  • Lv J  et al. Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages. Cell Discov 7:24 (2021). PubMed: 33850112
  • Castroflorio E  et al. The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. Cell Mol Life Sci 78:3503-3524 (2021). PubMed: 33340069
  • Bai L  et al. Simvastatin accelerated motoneurons death in SOD1G93A mice through inhibiting Rab7-mediated maturation of late autophagic vacuoles. Cell Death Dis 12:392 (2021). PubMed: 33846297
  • Salvany S  et al. Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury. Glia 69:1216-1240 (2021). PubMed: 33386754
View all Publications for this product

客户评价及客户问答

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1-10 of 23 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain cortex)
Permeabilization
Yes - 0.3% Triton X-100 in TBS for 1 h at room temperature
Specification
Brain cortex
Blocking step
10% normal goat serum (NGS) and 1% BSA in TBS for 1 h at RT as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 03 2022

Western blot abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 10 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Zebrafish kidney stromal cell line (ZKS))
Permeabilization
Yes - 0.3% Triton X100 in PBS pH 7.4
Specification
Zebrafish kidney stromal cell line (ZKS)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 24 2021

Western blot abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
HEK293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Nov 17 2020

Western blot abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 16 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB7 antibody [EPR7589]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblast)
Permeabilization
Yes - 0.1% Triton in PBS (5 mins)
Specification
Fibroblast
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 11 2020

Immunocytochemistry abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Hela cells)
Permeabilization
Yes - 0.1% triton
Specification
Hela cells
Blocking step
FBS as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 06 2020

Western blot abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293FT cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Treatment
NTC and Rab7A siRNA
Specification
HEK293FT cells
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 06 2020

Western blot abreview for Anti-RAB7 antibody [EPR7589]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293RT, HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
HEK293RT, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 31 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB7 antibody [EPR7589]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.1% triton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 15 2018

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