Anti-Rab5抗体- Drosophila Early Endosome Marker (ab31261)


  • 产品名称
    Anti-Rab5抗体- Drosophila Early Endosome Marker
    参阅全部 Rab5 一抗
  • 描述
    兔多克隆抗体to Rab5 - Drosophila Early Endosome Marker
  • 宿主
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.

    (Peptide available as ab32489.)

  • 阳性对照
    • This antibody gave a positive signal in Schneider L2 (Fruit fly (Drosophila melanogaster)) Whole Cell Lysate.



Our Abpromise guarantee covers the use of ab31261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20404143
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).


  • 功能
    Required for the fusion of plasma membranes and early endosomes.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rab family.
  • 细胞定位
    Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • 别名
    • RAB 5 antibody
    • RAB 5A antibody
    • RAB5A antibody
    • RAB5A member RAS oncogene family antibody
    • RAB5A_HUMAN antibody
    • RAS associated protein RAB5A antibody
    • Ras related protein Rab 5A antibody
    • Ras-related protein Rab-5A antibody
    see all


  • ab31261 staining 3rd instar wing imaginal disc cells of fruit fly (Drosophila melanogaster).  Cells were formaldehyde fixed and blocked with 5 % serum for 2 hours at 22°C prior to incubating with the primary antibody, diluted 1/500, for 16 hours at 4°C.  A Cy3® conjugated donkey anti-rabbit IgG (H+L) antibody, diluted 1/500, was used as the secondary.

    In the image, the nuclei of some cells are marked by GFP (green).  The punctate, non-nuclear staining pattern is indicative of endosomes (similar to other endosomal markers such as Hrs).

    See Abreview

  • Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes

    ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.
  • ab31261 staining Rab5 in Drosophila S2 cells by Immunocytochemistry/ Immunofluorescence.
    S2 cells were fixed in 4% paraformaldehyde for 10 minutes and then permeablized by incubating with 0.2% Triton X-100 in PBS for 5 minutes at room temperature. After blocking, cells were incubated overnight with ab31261 diluted in PBS containing 10% horse serum and 0.2% Triton X-100. A Fluorescein-conjugated anti-rabbit IgG was used as the secondary.


This product has been referenced in:
  • Donohoe CD  et al. Atf3 links loss of epithelial polarity to defects in cell differentiation and cytoarchitecture. PLoS Genet 14:e1007241 (2018). Read more (PubMed: 29494583) »
  • Jin EJ  et al. Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Curr Biol 28:1027-1038.e4 (2018). Read more (PubMed: 29551411) »

See all 32 Publications for this product


Thank you for contacting us and your interest in our products. The immunogen sequence used to raise ab31261 Anti-Rab5 antibody is proprietary information. However, in order to aid your research I am able to share it with you. Please do not share o...

Read More

Thank you for contacting us. Because we carry over 77,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarant...

Read More