Anti-Rab5A抗体[3A4] - Early Endosome Marker (ab66746)
Key features and details
- Mouse monoclonal [3A4] to Rab5A - Early Endosome Marker
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Rab5A抗体[3A4] - Early Endosome Marker
参阅全部 Rab5A 一抗 -
描述
小鼠单克隆抗体[3A4] to Rab5A - Early Endosome Marker -
宿主
Mouse -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Rab5a aa 1-250.
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阳性对照
- WB: Extracts of mouse and human brain. ICC/IF: U87MG cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
3A4 -
骨髓瘤
Sp2/0 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab66746于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
靶标
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功能
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. -
序列相似性
Belongs to the small GTPase superfamily. Rab family. -
细胞定位
Cell membrane. Early endosome membrane. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). - Information by UniProt
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数据库链接
- Entrez Gene: 5868 Human
- SwissProt: P20339 Human
- Unigene: 475663 Human
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别名
- RAB5A antibody
- RAB5A_HUMAN antibody
- Ras-related protein Rab-5A antibody
图片
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.This image was produced using the ascites version of this antibody.
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Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + mouse brain at 45 µg
Secondary
goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 24 kDa -
Anti-Rab5A antibody [3A4] - Early Endosome Marker (ab66746) at 1/500 dilution + extracts of mouse brain at 45 µg
Secondary
HRP-conjugated goat anti-mouse
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 24 kDaThis image was produced using the ascites version of this antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab66746 被引用在 9 文献中.
- Patel M et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. Br J Cancer 126:1704-1714 (2022). PubMed: 35173303
- Tang Y et al. The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles. Acta Pharm Sin B 12:2043-2056 (2022). PubMed: 35847504
- Gorji-Bahri G et al. RAB5A effect on metastasis of hepatocellular carcinoma cell line via altering the pro-invasive content of exosomes. Exp Mol Pathol 120:104632 (2021). PubMed: 33831402
- Gorji-Bahri G et al. RAB5A is associated with genes involved in exosome secretion: Integration of bioinformatics analysis and experimental validation. J Cell Biochem 122:425-441 (2021). PubMed: 33225526
- Polla DL et al. Biallelic variants in TMEM222 cause a new autosomal recessive neurodevelopmental disorder. Genet Med 23:1246-1254 (2021). PubMed: 33824500
- Nanou A et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis. Cell Rep 35:109168 (2021). PubMed: 34038728
- Huang HW & Ryoo HD Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival. PLoS Genet 17:e1009551 (2021). PubMed: 34714826
- Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun 9:3744 (2018). PubMed: 30218067
- Del Cid-Pellitero E et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). PubMed: 28408870