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Our Abpromise guarantee covers the use of ab66746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 2-5µg for 106 cells.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab66746 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab66746 staining Rab5 in the U87MG human cell line at a dilution of 1/100. The cells were fixed before staining with a mixture of acetone and methanol, 1:1. DAPI was used fhor nuclear staining.
Flow cytometry analysis of ab66746 staining Rab5 in HeLa cells. Primary antibody was used at a concentration of 2-5ug per 1 X 106 cells. The secondary antibody used was an Alexa fluor 488® conjugated goat anti-mouse IgG.
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