重组Anti-QKI抗体[EPR7306] (ab126742)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7306] to QKI
- Suitable for: WB, IP, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-QKI抗体[EPR7306]
参阅全部 QKI 一抗 -
描述
兔单克隆抗体[EPR7306] to QKI -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human QKI aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q96PU8 -
阳性对照
- WB: HAP1, Neuro 2a, HeLa and K562 whole cell lysates. IP: HeLa whole cell lysate. ICC/IF: Neuro-2a cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 4.30 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7306 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126742于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).
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IP |
1/10 - 1/100.
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ICC/IF |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa). |
IP
1/10 - 1/100. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. -
组织特异性
Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients. -
序列相似性
Contains 1 KH domain. -
结构域
The KH domain and the Qua2 region are involved in RNA binding. -
翻译后修饰
Methylated by PRMT1.
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 9444 Human
- Entrez Gene: 19317 Mouse
- Omim: 609590 Human
- SwissProt: Q96PU8 Human
- SwissProt: Q9QYS9 Mouse
- Unigene: 510324 Human
- Unigene: 130694 Mouse
- Unigene: 384135 Mouse
see all -
别名
- DKFZp586I0923 antibody
- HKQ antibody
- Homolog of mouse quaking QKI KH domain RNA binding protein antibody
see all
图片
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All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : QKI knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : THP-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDaLanes 1 - 4: Merged signal (red and green). Green - ab126742 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab126742 was shown to specifically react with in wild-type HAP1 cells as signal was lost in QKI knockout cells. Wild-type and QKI knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab126742 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling QKI with Purified ab126742 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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ab126742 (purified) at 1:30 dilution (2µg) immunoprecipitating QKI in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab126742 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126742 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/2000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution (unpurified)
Lane 1 : Neuro 2a cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab126742 被引用在 16 文献中.
- Derbis M et al. Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats. Nat Commun 12:1265 (2021). PubMed: 33627639
- Chen X et al. QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function. Nat Commun 12:89 (2021). PubMed: 33397958
- Xiong M et al. LncRNA DANCR represses Doxorubicin-induced apoptosis through stabilizing MALAT1 expression in colorectal cancer cells. Cell Death Dis 12:24 (2021). PubMed: 33414433
- Shen Y et al. G protein-coupled oestrogen receptor promotes cell growth of non-small cell lung cancer cells via YAP1/QKI/circNOTCH1/m6A methylated NOTCH1 signalling. J Cell Mol Med 25:284-296 (2021). PubMed: 33237585
- Konieczny P et al. Cyclic mismatch binding ligands interact with disease-associated CGG trinucleotide repeats in RNA and suppress their translation. Nucleic Acids Res 49:9479-9495 (2021). PubMed: 34358321