重组Anti-PDHB抗体[EPR11097(B)] (ab155996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11097(B)] to PDHB
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PDHB抗体[EPR11097(B)]
参阅全部 PDHB 一抗 -
描述
兔单克隆抗体[EPR11097(B)] to PDHB -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cyt, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human lung cancer, Mouse spleen and Rat colon tissue; ICC/IF: HepG2 cells; Flow Cyt: HeLa cells. WB: A375 and HeLa whole cell lysates. Mouse spleen and kidney lysates. Rat kidney lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11097(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-PDHB antibody [EPR11097(B)] (ab208255)
- Alexa Fluor® 647 Anti-PDHB antibody [EPR11097(B)] (ab210028)
- Alexa Fluor® 405 Anti-PDHB antibody [EPR11097(B)] (ab210029)
- Alexa Fluor® 594 Anti-PDHB antibody [EPR11097(B)] (ab211838)
- Anti-PDHB antibody [EPR11097(B)] - BSA and Azide free (ab249239)
- Alexa Fluor® 568 Anti-PDHB antibody [EPR11097(B)] (ab312467)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50 - 1/250.
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Flow Cyt |
1/20.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
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|
IHC-P |
1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50- 1/100. |
说明 |
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ICC/IF
1/50 - 1/250. |
Flow Cyt
1/20. |
WB
1/10000 - 1/50000. Predicted molecular weight: 35 kDa. |
IHC-P
1/1500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50- 1/100. |
靶标
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功能
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). -
疾病相关
Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy). -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5162 Human
- Entrez Gene: 68263 Mouse
- Entrez Gene: 289950 Rat
- Omim: 179060 Human
- SwissProt: P11177 Human
- SwissProt: Q9D051 Mouse
- SwissProt: P49432 Rat
- Unigene: 161357 Human
see all -
别名
- DKFZp564K0164 antibody
- mitochondrial antibody
- ODPB_HUMAN antibody
see all
图片
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All lanes : Anti-PDHB antibody [EPR11097(B)] (ab155996) at 1/10000 dilution (Purified)
Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Rat kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling PDHB with purified ab155996 at 1/1500 dilution (0.08 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PDHB with purified ab155996 at 1/50 dilution (2.5 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PDHB with purified ab155996 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling PDHB with purified ab155996 at 1/1500 dilution (0.08 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling PDHB with purified ab155996 at 1/1500 dilution (0.08 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab155996 被引用在 10 文献中.
- Luo G et al. Acetyl-CoA Deficiency Is Involved in the Regulation of Iron Overload on Lipid Metabolism in Apolipoprotein E Knockout Mice. Molecules 27:N/A (2022). PubMed: 35956917
- Wang G et al. LncRNA MEG3 promotes endoplasmic reticulum stress and suppresses proliferation and invasion of colorectal carcinoma cells through the MEG3/miR-103a-3p/PDHB ceRNA pathway. Neoplasma 68:362-374 (2021). PubMed: 33118833
- Jabri Y et al. Cell-Type-Specific Complement Profiling in the ABCA4-/- Mouse Model of Stargardt Disease. Int J Mol Sci 21:N/A (2020). PubMed: 33187113
- Lee J et al. The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity. PLoS One 15:e0243489 (2020). PubMed: 33370314
- Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989
- Pauly D et al. Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina. Cell Rep 29:2835-2848.e4 (2019). PubMed: 31775049
- Groehler A et al. Oxidative cross-linking of proteins to DNA following ischemia-reperfusion injury. Free Radic Biol Med 120:89-101 (2018). Rat . PubMed: 29540307
- Xu Z et al. Proteomics Analysis Reveals Abnormal Electron Transport and Excessive Oxidative Stress Cause Mitochondrial Dysfunction in Placental Tissues of Early-Onset Preeclampsia. Proteomics Clin Appl 12:e1700165 (2018). PubMed: 29676857
- Jin X et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). PubMed: 28487965
- Mazar J et al. MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells. Mol Cell Biol 36:1090-108 (2016). PubMed: 26787841