Anti-PDHA1 (phospho S293)抗体(ab92696)
Key features and details
- Rabbit polyclonal to PDHA1 (phospho S293)
- Suitable for: WB, ICC/IF, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PDHA1 (phospho S293)抗体
参阅全部 PDHA1 一抗 -
描述
兔多克隆抗体to PDHA1 (phospho S293) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, ELISAmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Orangutan -
免疫原
Synthetic peptide within Human PDHA1 aa 250-350 (phospho S293) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab104429) -
阳性对照
- This antibody gave a positive signal in Human liver tissue lysate as well as the following whole cell lysates: HeLa; HEK293; HepG2. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92696于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (9) |
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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ICC/IF | (2) |
Use a concentration of 1 - 5 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. |
ELISA
Use at an assay dependent concentration. |
靶标
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功能
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). -
组织特异性
Ubiquitous. -
疾病相关
Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 407109 Cow
- Entrez Gene: 5160 Human
- Entrez Gene: 18597 Mouse
- Entrez Gene: 29554 Rat
- Omim: 300502 Human
- SwissProt: A7MB35 Cow
- SwissProt: P08559 Human
- SwissProt: P35486 Mouse
see all -
别名
- ODPA_HUMAN antibody
- PDH antibody
- PDHA antibody
see all
图片
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ab92696 staining PDHA1 (phospho S293) in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab92696 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-PDHA1 (phospho S293) antibody (ab92696) at 1 µg/ml
All lanes : HeLa Whole Cell Lysate + Calyculin A (30 nM for 20 min)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit (green) and goat anti-mouse (red) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDaThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour and then treated with either buffer (lane 1) or alkaline phosphatase (lane 2), before being incubated with ab92696 overnight at 4°C. Antibody binding was detected using IR-labelled goat anti-rabbit (green) and goat anti-mouse (Red) at 1:10,000 dilution for one hour at room temperature before imaging.
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IHC image of PDHA1 (phospho S293) staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92696, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Anti-PDHA1 (phospho S293) antibody (ab92696) at 1/1000 dilution + Human vascular smooth muscle cell whole cell lysate at 25 µg
Secondary
Polyclonal goat anti-rabbit IRDye® 800CW at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 5 minutes
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Serially diluted ab92696 was bound to immobilised Phospho peptide (S293) - or Control peptide (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG
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ab92696 staining PDHA1 (phospho S293) in Human HUVEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour . A CF488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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All lanes : Anti-PDHA1 (phospho S293) antibody (ab92696) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Modified blocking peptide at 1 µg/ml
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Modified blocking peptide at 1 µg/ml
Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Modified blocking peptide at 1 µg/ml
Lane 8 : Human liver tissue lysate - total protein (ab29889) with Modified blocking peptide at 1 µg/ml
Lane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Non-modified blocking peptide at 1 µg/ml
Lane 10 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Non-modified blocking peptide at 1 µg/ml
Lane 11 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Non-modified blocking peptide at 1 µg/ml
Lane 12 : Human liver tissue lysate - total protein (ab29889) with Non-modified blocking peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (67)
ab92696 被引用在 67 文献中.
- Sargsyan A et al. HGFAC is a ChREBP-regulated hepatokine that enhances glucose and lipid homeostasis. JCI Insight 8:N/A (2023). PubMed: 36413406
- Han A et al. Pyruvate dehydrogenase inactivation causes glycolytic phenotype in BAP1 mutant uveal melanoma. Oncogene 41:1129-1139 (2022). PubMed: 35046531
- Uchenunu O et al. Mitochondrial complex IV defects induce metabolic and signaling perturbations that expose potential vulnerabilities in HCT116 cells. FEBS Open Bio 12:959-982 (2022). PubMed: 35302710
- Sebastian C et al. A non-dividing cell population with high pyruvate dehydrogenase kinase activity regulates metabolic heterogeneity and tumorigenesis in the intestine. Nat Commun 13:1503 (2022). PubMed: 35314684
- Emtenani S et al. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. EMBO J 41:e109049 (2022). PubMed: 35319107