Pyrophosphate Assay试剂盒II (Fluorometric) (ab179836)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate
- Sensitivity: 1 µM
概述
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产品名称
Pyrophosphate Assay试剂盒II (Fluorometric)
参阅全部 Pyrophosphate 试剂盒 -
检测方法
Fluorescent -
样品类型
Serum, Plasma, Cell Lysate, Tissue Lysate -
检测类型
Quantitative -
灵敏度
>= 1 µM -
种属反应性
与反应: Mammals, Other species -
产品概述
Pyrophosphate (PPi) are produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.
Pyrophosphate Assay Kit II (Fluorometric) (ab179836) provides the most robust spectrophotometric method for the measurement of pyrophosphate. It uses our proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Our assay is much easier and more robust than enzyme-coupling pyrophosphate methods, which require at least two enzymes for their pyrophosphate detections. Due to its direct measurement of pyrophosphate, this kit is ideal for screening inhibition or activities of enzymes that consume or generate pyrophosphate with enhanced selectivity to pyrophosphate. The assay is an optimized mix-and-read assay and can be performed in a convenient 96-well or 384-well microtiter-plate format. The kit provides all the essential components for assaying pyrophosphate.
Assay time: 10-30 minutes
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说明
This product measures PPi formation at Ex/Em = 370/470 nm.
If your instrument has filters for Ex/Em = 316/456 nm, we recommend using Pyrophosphate Assay Kit (Fluorometric) (ab112155).
Samples such as plasma and serum might need a deproteinization step with spin columns
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 200 tests Assay Buffer 1 x 25ml DMSO 1 x 100µl PPi Sensor 1 vial Pyrophosphate Standard (50mM) 1 x 1ml -
研究领域
图片
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Pyrophosphate measured in mouse tissue lysates showing quantities (nanomoles) per microgram of protein tested. The lysates were tested in the range of 2-50 microgram of proteins per mL, data shown from duplicates, +/- SD.
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Pyrophosphate measured in cell lysates showing quantity (micromoles) per 1 million cells tested.
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Pyrophosphate measured in biological fluids showing concentration (millimolar). The samples were tested in the dilution range of 1:200-1:5000.
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Pyrophosphate, ATP and phosphate dose responses were measured with the Abcam Pyrophosphate Assay Kit II (Fluorometric) (ab179836) in a solid black 96-well plate using a fluorescence microplate reader. As low as 1 µM (100 picmoles/well) pyrophosphate can be detected with 10 minutes incubation.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab179836 被引用在 1 文献中.
- Xiong L et al. Osteoblastic Lrp4 promotes osteoclastogenesis by regulating ATP release and adenosine-A2AR signaling. J Cell Biol 216:761-778 (2017). PubMed: 28193701