Key features and details
- Rabbit polyclonal to PYK2 (phospho Y881)
- Suitable for: WB
- Reacts with: Chicken
- Isotype: IgG
产品名称Anti-PYK2 (phospho Y881)抗体
参阅全部 PYK2 一抗
描述兔多克隆抗体to PYK2 (phospho Y881)
特异性Some cross-reactivity with Focal Adhesion Kinase phosphorylated at tyrosine 925 (the corresponding Grb 2 binding site) may be observed in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 in the same cell system.
经测试应用适用于: WBmore details
预测可用于: Mouse, Rat
Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 881.
- WB: Chicken embryo fibroblasts plated on fibronectin
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from rabbit serum by epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosine 881.
The Abpromise guarantee
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK 2 (phospho) antibody panel or CEF whole cell lysate, positive / negative for PYK 2 (pair).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
组织特异性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
翻译后修饰Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
细胞定位Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
- Information by UniProt
- CADTK antibody
- CAK-beta antibody
- CAKB antibody
All lanes : Anti-PYK2 (phospho Y881) antibody (ab4801) at 1/1000 dilution
Lane 1 : Mock-transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 2 : Only expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 3 : Pyk2 (pY^881)NP expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 4 : FAK (pY^925) expression vector transfected- Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 5 : Generic pY expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 6 : Pyk2 (pY^881)P - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Predicted band size: 116 kDa
ab4801 被引用在 1 文献中.
- Tsai CS et al. Thrombomodulin regulates monocye differentiation via PKCd and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep 6:38421 (2016). WB ; Human . PubMed: 27910925