Key features and details
- Rabbit polyclonal to PYK2 (phospho Y580)
- Suitable for: WB
- Reacts with: Rat, Chicken, Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: PBS, 50% Glycerol
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from rabbit serum by epitope-specific chromatography. Any reactivity towards the non-tyrosine phosphorylated PYK 2 protein, the corresponding phosphotyrosine (577) of the Focal Adhesion Kinase protein, or generic phosphotyrosine has been eliminated through a series of preadsorption steps.
Our Abpromise guarantee covers the use of ab4806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 125 kDa.|
功能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
组织特异性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
翻译后修饰Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
细胞定位Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
- Information by UniProt
- CADTK antibody
- CAK-beta antibody
- CAKB antibody
Cell extracts prepared from chick embryo fibroblasts expressing PYK 2 and plated on fibronectin were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 0.5
µg/mL ab4806, following prior incubation in the absence (none) or presence of the peptide immunogen, the nonphosphopeptide corresponding to the PYK 2 phosphopeptide, the phosphopeptide corresponding to tyrosine 577 on FAK, the phosphopeptide corresponding to tyrosine 579 on PYK 2, and a generic phosphotyrosine containing peptide. After washing, membranes were incubated with goat F(ab’)2 anti rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of the ab4806 antibody for this phosphorylated residue. Cell extracts prepared from chick embryo
Extracts prepared from CEFs (1) or CEFs expressing PYK2 and plated on fibronectin (2-7) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with PYK2 (phospho Y580) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), the phosphopeptide corresponding to PYK2 (phospho Y579) (4), a phosphopeptide derived from the corresponding region of FAK (5), a generic phosphotyrosine-containing peptide (6) or, the phosphopeptide immunogen (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method.
The data show that only the peptide corresponding to PYK2 (phospho Y580) completely blocks the antibody signal, thereby demonstrating the specificity of the antibody.