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Signal Transduction Protein Phosphorylation Tyrosine Kinases FAK / PYK

Anti-PYK2 (phospho Y402)抗体(ab4800)

  • Datasheet
Submit a review Q&A (3)References (8)

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Western blot - Anti-PYK2 (phospho Y402) antibody (ab4800)
  • Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800)

Key features and details

  • Rabbit polyclonal to PYK2 (phospho Y402)
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Chicken, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-PYK2 (phospho Y402)抗体
    参阅全部 PYK2 一抗
  • 描述

    兔多克隆抗体to PYK2 (phospho Y402)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide (Human) derived from the region of human Pyk 2 that contains tyrosine 402. The sequence is conserved in human and rat.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from rabbit serum by epitope specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosine 402.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • FAK / PYK
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PYK2 protein (ab152380)

应用

Our Abpromise guarantee covers the use of ab4800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK2 peptide - phospho Y402 (phospho and non-phospho pair) or CEF whole cell lysate, positive / negative for PYK 2 (pair).
    ICC/IF: Use at an assay dependent dilution from (PubMed:17325207).
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能

      Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
    • 组织特异性

      Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
    • 序列相似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰

      Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
    • 细胞定位

      Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Target information above from: UniProt accession Q14289 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 421998 Chicken
      • Entrez Gene: 2185 Human
      • Entrez Gene: 19229 Mouse
      • Entrez Gene: 50646 Rat
      • Omim: 601212 Human
      • SwissProt: Q14289 Human
      • SwissProt: Q9QVP9 Mouse
      • SwissProt: P70600 Rat
      • Unigene: 491322 Human
      • Unigene: 21613 Mouse
      • Unigene: 11025 Rat
      see all
    • 别名

      • CADTK antibody
      • CAK-beta antibody
      • CAKB antibody
      • CAKbeta antibody
      • Calcium regulated non receptor proline rich tyrosine kinase antibody
      • Calcium-dependent tyrosine kinase antibody
      • Cell adhesion kinase beta antibody
      • E430023O05Rik antibody
      • EC 2.7.10.2 antibody
      • FADK 2 antibody
      • FADK2 antibody
      • FAK2 antibody
      • FAK2_HUMAN antibody
      • Focal adhesion kinase 2 antibody
      • MGC124628 antibody
      • PKB antibody
      • Proline-rich tyrosine kinase 2 antibody
      • Protein kinase B antibody
      • Protein Tyrosine Kinase 2 Beta antibody
      • Protein-tyrosine kinase 2-beta antibody
      • PTK antibody
      • PTK2B antibody
      • PTK2B protein tyrosine kinase 2 beta antibody
      • PYK2 antibody
      • RAFTK antibody
      • RAFTK2 antibody
      • Related adhesion focal tyrosine kinase antibody
      see all

    图片

    • Western blot - Anti-PYK2 (phospho Y402) antibody (ab4800)
      Western blot - Anti-PYK2 (phospho Y402) antibody (ab4800)
      Peptide Competition: Extracts prepared from CEF cells expressing Pyk2 and plated on fibronectin were resolved by SDS-PAGE on a 10% Tris glycine gel. The proteins were then transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4800 antibody, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to phosphopeptide immunogen (2), a generic phosphotyrosine containing peptide (3), the phosphopeptide corresponding to tyrosine 397 on FAK (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of ab4800 antibody for this phosphorylated residue. Peptide Competition: Extracts p
    • Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800)
      Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800)
      Figure shows Pyk2 staining: Normal mouse mammary (NmuMG) cells were treated with TGFbeta and then grown to confluence, fixed and permeabilized. ab4800 was identified using a selective PSSA followed by incubation with a Cy3-conjugated anti-rabbit secondary antibody (red). F actin was visualized with Oregon Green-conjugated phalloiden (green) and paxillin staining is indicated in blue. The merged image indicates colocalization (purple) of ab4800, F-actin and paxillin in focal adhesion structures.

    实验方案

    • Peptide Competition Experiment
    • Western blotting protocol

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (8)

    发表研究结果有使用 ab4800?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4800 被引用在 8 文献中.

    • Sai C  et al. Cyclin Dependent Kinase 1 (CDK1) Activates Cardiac Fibroblasts via Directly Phosphorylating Paxillin at Ser244. Int Heart J 60:374-383 (2019). PubMed: 30745530
    • Wang Q  et al. Small molecule FAK activator promotes human intestinal epithelial monolayer wound closure and mouse ulcer healing. Sci Rep 9:14669 (2019). PubMed: 31604999
    • Tiede S  et al. The FAK inhibitor BI 853520 exerts anti-tumor effects in breast cancer. Oncogenesis 7:73 (2018). PubMed: 30237500
    • Haidari M  et al. Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-ß-dependent vascular endothelial cadherin tyrosine phosphorylation. Cardiovasc Diabetol 13:112 (2014). Human . PubMed: 25027642
    • Haidari M  et al. Atorvastatin preserves the integrity of endothelial adherens junctions by inhibiting vascular endothelial cadherin tyrosine phosphorylation. Exp Cell Res 318:1673-84 (2012). WB ; Human . PubMed: 22659162
    • Xie H  et al. Silencing or Amplification of Endocannabinoid Signaling in Blastocysts via CB1 Compromises Trophoblast Cell Migration. J Biol Chem 287:32288-97 (2012). WB . PubMed: 22833670
    • Suo L  et al. Protocadherin clusters and cell adhesion kinase regulate dendrite complexity through Rho GTPase. J Mol Cell Biol : (2012). PubMed: 22730554
    • Schober M  et al. Focal adhesion kinase modulates tension signaling to control actin and focal adhesion dynamics. J Cell Biol 176:667-80 (2007). PubMed: 17325207

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    Phone call requesting information on if the Anti-PYK2 (phospho Y402) antibody (ab4800) is likely to cross react with other phospho-proteins.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 19 2013

    Answer

    Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.

    As we discussed over the phone, I have looked into how the Anti-PYK2 (phospho Y402) antibodies ab4800 and ab131543 have been characterized for their potential cross-reactivity with other phospho-proteins.

    No specific experiments have been carried out to ascertain the cross-reactivity of either of these antibodies. However, the western results are based on total cell lysates which contains thousands of other proteins many of which are phosphorylated. As the Western blot images for these antibodies show, only one band was detected in the molecular range from 37 kDa up to 250 kDa, there is no other visible band.

    In addition, with ab4800 the target band was competed out with the phosphorylated immunogen peptide indicating the band we see is the targe Pyk2 pY402. This supports at least in the cells (primary CDF cells, and SDF-1a treated Reh cells) we studied we did not see any cross reactivity with other proteins (phosphorylated or not phosphorylated).

    From blast search of the immunogens used, the area does not seem to share homology with any other protein (that have been added to this databank) suggesting there should not be any cross-reactivity observed.

    Read More

    Abcam Scientific Support

    回复于 Feb 19 2013

    Question

    Hi,   I have technical questions regarding 2 of your products: PYK2 protein (Active), cat. no ab60878, and PYK2 protein (His tag), cat. no ab42622.   I need to know the phosphorylation site of these 2 proteins. More specifically, I need to know if these proteins are phosphorylated at Tyrosine 402.  

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Thank you for contacting us. I do want to apologize for the tardiness of this response.

    I have been in contact with the originator labs for ab42622 and ab60878 PYK2 proteins, in regards to whether these products are phosphorylated at Y402. Unfortunately neither group was able to confirm whether Y402 is phosphorylated at this site and each recommended that phophorylation state may be determined by performing a western blot with a phospho specific antibody such as ab4800 (https://www.abcam.com/PYK2-phospho-Y402-antibody-ab4800.html).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Abcam Scientific Support

    回复于 May 18 2012

    Question

    I am interested in ab4800, the Pyk2 phospho Y402 antibody. We would like to use it on mouse pituitary glands. Can you tell me if this antibody works with paraffin-embedded sections? If not under what conditions might it work in tissues rather than cells (your references on the web site are all cells)?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2004

    Answer

    Thank you for your enquiry. Ab4800 was tested in IHC cryostat sections in the following reference: Melendez J. et al (2002) J. Biol. Chem. 277(47):45203-45210. The authors prepared mouse heart sections fixed in 4% paraformaldehyde/PBS overnight at 4 degrees C. The next day, the hearts were subjected to progressive sucrose gradient from 10%, 20%, 30% at 4 degrees C for 1 hour at each step. Embedded in Tisse-Tek, frozen in melting isopentane floated above liquid nitrogen, and frozen blocks were stored at -80 degrees C Section 7 microns thick were mounted on Superfrost slides (Fisher Scientific Co), allowed to dry, and then stored at 4 degrees C until used within 1 week. For labeling, primary antibodies were applied to the sections and left overnight. The next day, the sections were washed, secondary antibodies applied to the sections for 1 hour, washed again, and then mounted in Vectashield (Vecyor Laboratories). Primary antibodies were detected by fluorescent secondary goat anti-rabbit IgG. We recommend using the antibody at ~1:50 dilution to 1:100 dilution. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Apr 01 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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