Key features and details
- Rabbit polyclonal to PYK2 (phospho Y402)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
产品名称Anti-PYK2 (phospho Y402)抗体
参阅全部 PYK2 一抗
描述兔多克隆抗体to PYK2 (phospho Y402)
特异性ab131543 detects endogenous levels of PYK2 only when phosphorylated at Tyrosine 402.
经测试应用适用于: WB, IHC-P, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Human
- HeLa cells; Jurkat whole cell lysate (ab7899) (PMA-treated); Human breast carcinoma tissue.
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS without Mg2+ and Ca2+
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab131543 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 116 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
功能Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
组织特异性Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
翻译后修饰Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
细胞定位Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
- Information by UniProt
- CADTK antibody
- CAK-beta antibody
- CAKB antibody
All lanes : Anti-PYK2 (phospho Y402) antibody (ab131543) at 1/500 dilution
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with PMA
Predicted band size: 116 kDa
Immunoflourescent analysis of methanol-fixed HeLa cells labelling PYK2 (phospho-Y402) with ab131543 at 1/100 dilution.
Immunohistochemical analysis of Human breast carcinoma tissue labelling PYK2 (phospho Y402) with ab131543 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
ab131543 被引用在 2 文献中.