Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 369-419 of Human PYGO1 (NP_056432.1).
Whole cell lysate from HeLa and 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
Use at 2-5 µg/mg of lysate.
Involved in signal transduction through the Wnt pathway.
Contains 1 PHD-type zinc finger.
Information by UniProt
PYGO 1 antibody
Western blot - Anti-PYGO1 antibody (ab95072)
All lanes : Anti-PYGO1 antibody (ab95072) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 45 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-PYGO1 antibody (ab95072)
Detection of PYGO1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95072 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"