Anti-PYGL抗体(ab198268)
Key features and details
- Rabbit polyclonal to PYGL
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PYGL抗体
参阅全部 PYGL 一抗 -
描述
兔多克隆抗体to PYGL -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment (GST-tag) corresponding to Human PYGL (C terminal). BC082229
Database link: P06737 -
阳性对照
- 231 and PC3 cell lysates. Human thyroid cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198268于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/30 - 1/150.
|
WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 97 kDa.
|
说明 |
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IHC-P
1/30 - 1/150. |
WB
1/500 - 1/2000. Predicted molecular weight: 97 kDa. |
靶标
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功能
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. -
疾病相关
Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6) [MIM:232700]. A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. -
序列相似性
Belongs to the glycogen phosphorylase family. -
翻译后修饰
Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A. - Information by UniProt
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数据库链接
- Entrez Gene: 5836 Human
- Entrez Gene: 110095 Mouse
- Entrez Gene: 64035 Rat
- Omim: 613741 Human
- SwissProt: P06737 Human
- SwissProt: Q9ET01 Mouse
- SwissProt: P09811 Rat
- Unigene: 282417 Human
see all -
别名
- Glycogen phosphorylase antibody
- Glycogen phosphorylase L antibody
- Glycogen phosphorylase liver antibody
see all
图片
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All lanes : Anti-PYGL antibody (ab198268) at 1/400 dilution
Lane 1 : 231 cell lysate
Lane 2 : PC3 cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 20 seconds -
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PYGL with ab198268 at 1/35 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab198268 被引用在 9 文献中.
- Sadiku P et al. Neutrophils Fuel Effective Immune Responses through Gluconeogenesis and Glycogenesis. Cell Metab 33:411-423.e4 (2021). PubMed: 33306983
- Xu JH et al. Up-Regulation of Glycogen Synthesis and Degradation Enzyme Level Maintained Myocardial Glycogen in Huddling Brandt's Voles Under Cool Environments. Front Physiol 12:593129 (2021). PubMed: 33841168
- Xu J et al. Long noncoding RNA KCNMB2-AS1 promotes the development of esophageal cancer by modulating the miR-3194-3p/PYGL axis. Bioengineered 12:6687-6702 (2021). PubMed: 34516362
- Kawakubo-Yasukochi T et al. Hepatic glycogenolysis is determined by maternal high-calorie diet via methylation of Pygl and it is modified by oteocalcin administration in mice. Mol Metab 54:101360 (2021). PubMed: 34673295
- Liu C et al. Glycogen Phosphorylase: A Drug Target of Amino Alcohols in Echinococcus granulosus, Predicted by a Computer-Aided Method. Front Microbiol 11:557039 (2020). PubMed: 33329421
- Mantini G et al. Co-expression analysis of pancreatic cancer proteome reveals biology and prognostic biomarkers. Cell Oncol (Dordr) 43:1147-1159 (2020). PubMed: 32860207
- Hughey CC et al. Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation. Mol Metab 23:1-13 (2019). PubMed: 30850319
- Hughey CC et al. Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates. J Biol Chem 293:11944-11954 (2018). PubMed: 29891549
- Liang X et al. Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content. PLoS Biol 16:e2002907 (2018). PubMed: 29659562