存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab32894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 114 kDa).|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
组织特异性Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
序列相似性Contains 1 PUM-HD domain.
Contains 8 pumilio repeats.
结构域The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs.
细胞定位Cytoplasm. Cytoplasmic granule. Cytoplasm > perinuclear region. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Colocalizes with NANOS1 and SNAPIN in the perinuclear region of germ cells.
- Information by UniProt
- FLJ36528 antibody
- KIAA0235 antibody
- MGC138251 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human testis staining Pumilio 2 with ab32894 at 10 μg/ml
All lanes : Anti-Pumilio 2 antibody (ab32894) at 1 µg/ml
Lane 1 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - without blocking with the immunizing peptide
Lane 2 : Human Epstein-Barr virus immortilised lymphoblastoid lysate - blocking with the immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 114 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 65 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
NB: Some minor bands are resistant to blocking by the immunizing peptide.
Lane 1 : Anti-Pumilio 2 antibody (ab32894) at 0.5 µg/ml
Lane 2 : Anti-Pumilio 2 antibody (ab32894) at 1 mg/ml
Lane 3 : anti- DYKDDDDK Tag at 1/3000 dilution
Lanes 1 & 3 : HEK293 lysate overexpressing Human PUM2 with DYKDDDDK tag at 10 µg
Lane 2 : Mock Tansfected HEK293
Predicted band size: 114 kDa
Primary Incubation time 1 hour
ab32894 has not yet been referenced specifically in any publications.