Anti-Pumilio 1抗体(ab3717)

Submit a review Q&A (1)References (3)
Western blot - Anti-Pumilio 1 antibody (ab3717)

    Key features and details

    • Goat polyclonal to Pumilio 1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Pumilio 1抗体
      参阅全部 Pumilio 1 一抗

    • 描述

      山羊多克隆抗体to Pumilio 1

    • 宿主

      Goat

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant

    • 免疫原

      Synthetic peptide (Human) - which represented a portion of human Pumilio homolog 1 encoded in part by exons 5 and 6.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab3717 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).

    靶标

    图片

    • Western blot - Anti-Pumilio 1 antibody (ab3717)
      Western blot - Anti-Pumilio 1 antibody (ab3717)

      Sample: Hela cell total RIPA extract (10µg)

      Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

      CIP: Calf Intestinal Phosphatase

      Detection: ECL exposure for 1 minute.

      Sample: Hela cell total RIPA extract (10µg)

      Antibody: Affinity purified Goat anti-Pumilio 1 (ab3717) (diluted 1 / 30,000)

      CIP: Calf Intestinal Phosphatase

      Detection: ECL exposure for 1 minute.

    文献 (3)

    发表研究结果有使用 ab3717？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab3717 被引用在 3 文献中.

    • Sajek M  et al. PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues. Cell Mol Life Sci 76:147-161 (2019). PubMed: 30269240
    • Janecki DM  et al. SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma. Oncotarget 9:32466-32477 (2018). PubMed: 30197756
    • Marrero E  et al. Translational Regulation of Acetylcholinesterase by the RNA-binding Protein Pumilio-2 at the Neuromuscular Synapse. J Biol Chem 286:36492-9 (2011). IHC-Fr ; Mouse . PubMed: 21865157

    客户评价及客户问答

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    Question

    Customer's question: Do you know whether ab3717 cross reacts with Pum2. If you do not know, could you send me the peptide sequence to see if it is likely to cross react. Thank you

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    Answer

    ab3717 does not cross react with Pum2.

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