重组Anti-PTS/PTPS (phospho S19)抗体[EPR1517(2)] (ab109462)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1517(2)] to PTS/PTPS (phospho S19)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PTS/PTPS (phospho S19)抗体[EPR1517(2)]
参阅全部 PTS/PTPS 一抗 -
描述
兔单克隆抗体[EPR1517(2)] to PTS/PTPS (phospho S19) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HUVEC cell lysates; Human brain tissue; HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1517(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109462于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
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ICC/IF |
1/250 - 1/500.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin. -
通路
Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3. -
疾病相关
Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A) [MIM:261640]; also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet. -
序列相似性
Belongs to the PTPS family. -
翻译后修饰
Phosphorylation of Ser-19 is required for maximal enzyme activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5805 Human
- GenBank: NP_000308.1 Human
- Omim: 612719 Human
- SwissProt: Q03393 Human
- Unigene: 503860 Human
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别名
- 6 pyruvoyl tetrahydrobiopterin synthase antibody
- 6 pyruvoyl tetrahydropterin synthase antibody
- 6 pyruvoyltetrahydropterin synthase antibody
see all
图片
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All lanes : Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HUVEC cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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