Anti-PTPD1/PTPN21抗体(ab12550)
Key features and details
- Rabbit polyclonal to PTPD1/PTPN21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PTPD1/PTPN21抗体 -
描述
兔多克隆抗体to PTPD1/PTPN21 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12550于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
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说明 |
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WB
1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa). |
靶标
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相关性
PTPD1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. The predicted PTPD1 protein has 1,174 amino acids. Its overall structure is similar to the structures of PTPH1 and PTPMEG1 (PTPN4), with an N-terminal ezrin-like domain and a single PTP domain at the extreme C terminus. Northern blot analysis shows that PTPD1 is expressed abundantly in human placenta, lung, and skeletal muscle, and to a lesser extent in several other tissues. PTPD1 may have a role in liver regeneration and spermatogenesis. -
细胞定位
Cytoplasmic, Cytoskeleton -
数据库链接
- Entrez Gene: 11099 Human
- GenBank: NP_008970 Human
- Omim: 603271 Human
- SwissProt: Q16825 Human
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别名
- Protein tyrosine phosphatase D1 antibody
- Protein tyrosine phosphatase non receptor type 21 antibody
- PTPN 21 antibody
see all
图片
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All lanes : Anti-PTPD1/PTPN21 antibody (ab12550) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab12550 尚未被引用在任何文献中。