重组Anti-PTBP1抗体[EPR9049(B)] (ab134950)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9049(B)] to PTBP1
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PTBP1抗体[EPR9049(B)]
参阅全部 PTBP1 一抗 -
描述
兔单克隆抗体[EPR9049(B)] to PTBP1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human PTBP1 aa 350-450. The exact sequence is proprietary.
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阳性对照
- WB: HAP1, HeLa, Daudi, Jurkat and BXPC3 cell lysates. ICC/IF: HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
解离常数(KD)
KD = 8.10 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR9049(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. -
序列相似性
Contains 4 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5725 Human
- Omim: 600693 Human
- SwissProt: P26599 Human
- Unigene: 172550 Human
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别名
- 57 kDa RNA binding protein PPTB 1 antibody
- 57 kDa RNA-binding protein PPTB-1 antibody
- Heterogeneous nuclear ribonucleoprotein I antibody
see all
图片
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Immunofluorescent analysis of HeLa cells labelling PTBP1 with ab134950 at 1/100 dilution.
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All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PTBP1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDaLanes 1- 2: Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab134950 was shown to react with PTBP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type HeLa and PTBP1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab134950 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PTBP1 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDaLanes 1 - 2: Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab134950 was shown to specifically react with PTBP1 in wild-type HAP1 cells as signal was lost in PTBP1 knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. ab134950 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : BXPC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134950 被引用在 1 文献中.
- Zhang QX et al. Alternative splicing analysis showed the splicing factor polypyrimidine tract-binding protein 1 as a potential target in acute myeloid leukemia therapy. Neoplasma 69:1198-1208 (2022). PubMed: 36131606