Anti-PSMB5/MB1抗体(ab3330)
Key features and details
- Rabbit polyclonal to PSMB5/MB1
- Suitable for: WB, ICC
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-PSMB5/MB1抗体
参阅全部 PSMB5/MB1 一抗 -
描述
兔多克隆抗体to PSMB5/MB1 -
宿主
Rabbit -
特异性
Detects proteasome PSMB5/MB1.
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经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Cow, Human -
免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/500 - 1/5000. Detects a band of approximately 22 kDa.
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| ICC |
1/10 - 1/200.
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| 说明 |
|---|
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WB
1/500 - 1/5000. Detects a band of approximately 22 kDa. |
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ICC
1/10 - 1/200. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway. -
序列相似性
Belongs to the peptidase T1B family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 534640 Cow
- Entrez Gene: 5693 Human
- Entrez Gene: 19173 Mouse
- Omim: 600306 Human
- SwissProt: Q32KL2 Cow
- SwissProt: P28074 Human
- SwissProt: O55234 Mouse
- Unigene: 422990 Human
see all -
别名
- DKFZp459C139 antibody
- EC 3.4.25.1 antibody
- LMPX antibody
see all
图片
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Western blot - Anti-PSMB5/MB1 antibody (ab3330)All lanes : Anti-PSMB5/MB1 antibody (ab3330) at 1/1000 dilution
Lane 1 : NCI-H1299 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 3 : Mouse liver cell lysate
Lysates/proteins at 25 µg per lane.
Observed band size: 23 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of A431 (Human epidermoid carcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of BAEC (Bovine aortic endothelial cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of HeLa (Human epithelial adenocarcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (35)
ab3330 被引用在 35 文献中.
- Goetzke CC et al. Mitigated viral myocarditis in A/J mice by the immunoproteasome inhibitor ONX 0914 depends on inhibition of systemic inflammatory responses in CoxsackievirusB3 infection. Basic Res Cardiol 116:7 (2021). PubMed: 33523326
- Farini A et al. Defective dystrophic thymus determines degenerative changes in skeletal muscle. Nat Commun 12:2099 (2021). PubMed: 33833239
- Shrum S et al. The BK activator NS11021 partially protects rat kidneys from cold storage and transplantation-induced mitochondrial and renal injury. Arch Biochem Biophys 688:108410 (2020). PubMed: 32446891
- Farini A et al. PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy. Front Physiol 11:403 (2020). PubMed: 32508664
- Kröll-Hermi A et al. Proteasome subunit PSMC3 variants cause neurosensory syndrome combining deafness and cataract due to proteotoxic stress. EMBO Mol Med 12:e11861 (2020). PubMed: 32500975