Anti-PSMB5/MB1抗体(ab3330)
Key features and details
- Rabbit polyclonal to PSMB5/MB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-PSMB5/MB1抗体
参阅全部 PSMB5/MB1 一抗 -
描述
兔多克隆抗体to PSMB5/MB1 -
宿主
Rabbit -
特异性
Detects proteasome PSMB5/MB1.
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经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Cow, Human -
免疫原
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常规说明
Previously labelled as PSMB5.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | 1/500 - 1/5000. Detects a band of approximately 22 kDa. | |
IHC-P | Use a concentration of 4 µg/ml. | |
ICC/IF | 1/10 - 1/200. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway. -
序列相似性
Belongs to the peptidase T1B family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 534640 Cow
- Entrez Gene: 5693 Human
- Entrez Gene: 19173 Mouse
- Omim: 600306 Human
- SwissProt: Q32KL2 Cow
- SwissProt: P28074 Human
- SwissProt: O55234 Mouse
- Unigene: 422990 Human
see all -
别名
- DKFZp459C139 antibody
- EC 3.4.25.1 antibody
- LMPX antibody
see all
图片
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All lanes : Anti-PSMB5/MB1 antibody (ab3330) at 1/1000 dilution
Lane 1 : NCI-H1299 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 3 : Mouse liver cell lysate
Lysates/proteins at 25 µg per lane.
Observed band size: 23 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of A431 (Human epidermoid carcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of BAEC (Bovine aortic endothelial cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of HeLa (Human epithelial adenocarcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB5/MB1 antibody (ab3330)
ab3330 (4µg/ml) staining PSMB5/MB1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-PSMB5/MB1 antibody (ab3330)Image from Shen X et al., J Biol Chem, 286, 3618-29. Fig 4.; doi: 10.1074/jbc.M110.175810. Epub 2010 Nov 8.
Immunofluorescence analysis of RGC5 cells, staining PSMB5/MB1 with ab3330 at 1/100 dilution.
实验方案
文献 (29)
ab3330 被引用在 29 文献中.
- Shrum S et al. The BK activator NS11021 partially protects rat kidneys from cold storage and transplantation-induced mitochondrial and renal injury. Arch Biochem Biophys 688:108410 (2020). PubMed: 32446891
- Farini A et al. PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy. Front Physiol 11:403 (2020). PubMed: 32508664
- Studencka-Turski M et al. Molecular Insight Into the IRE1a-Mediated Type I Interferon Response Induced by Proteasome Impairment in Myeloid Cells of the Brain. Front Immunol 10:2900 (2019). PubMed: 31921161
- Shrum S et al. Specific BK Channel Activator NS11021 Protects Rat Renal Proximal Tubular Cells from Cold Storage-Induced Mitochondrial Injury In Vitro. Biomolecules 9:N/A (2019). PubMed: 31817165
- Parajuli N A Cycle of Altered Proteasome and Reactive Oxygen Species Production in Renal Proximal Tubular Cells. Toxicol Forensic Med 4:13-17 (2019). PubMed: 32149269