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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-PSMB5/MB1抗体(ab3330)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (30)

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Western blot - Anti-PSMB5/MB1 antibody (ab3330)
  • Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)
  • Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)
  • Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)

Key features and details

  • Rabbit polyclonal to PSMB5/MB1
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-PSMB5/MB1抗体
    参阅全部 PSMB5/MB1 一抗
  • 描述

    兔多克隆抗体to PSMB5/MB1
  • 宿主

    Rabbit
  • 特异性

    Detects proteasome PSMB5/MB1.

  • 经测试应用

    适用于: WB, ICCmore details
  • 种属反应性

    与反应: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse PSMB5/MB1 aa 243-259.
    Sequence:

    WIRVSSDNVADLHDKYS


    Database link: P28074
    (Peptide available as ab4946)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Previously labelled as PSMB5. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PSMB5/MB1 protein (ab101825)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3330于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
1/500 - 1/5000. Detects a band of approximately 22 kDa.
ICC
1/10 - 1/200.
说明
WB
1/500 - 1/5000. Detects a band of approximately 22 kDa.
ICC
1/10 - 1/200.

靶标

  • 功能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway.
  • 序列相似性

    Belongs to the peptidase T1B family.
  • 细胞定位

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P28074 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 534640 Cow
    • Entrez Gene: 5693 Human
    • Entrez Gene: 19173 Mouse
    • Omim: 600306 Human
    • SwissProt: Q32KL2 Cow
    • SwissProt: P28074 Human
    • SwissProt: O55234 Mouse
    • Unigene: 422990 Human
    • Unigene: 8911 Mouse
    see all
  • 别名

    • DKFZp459C139 antibody
    • EC 3.4.25.1 antibody
    • LMPX antibody
    • Macropain epsilon chain antibody
    • MB1 antibody
    • MGC104214 antibody
    • MGC118075 antibody
    • MGC134464 antibody
    • Multicatalytic endopeptidase complex epsilon chain antibody
    • Proteasome (prosome, macropain) subunit, beta type, 5 antibody
    • Proteasome beta 5 subunit antibody
    • Proteasome catalytic subunit 3 antibody
    • Proteasome chain 6 antibody
    • Proteasome epsilon chain antibody
    • Proteasome subunit beta type-5 antibody
    • Proteasome subunit MB1 antibody
    • Proteasome subunit X antibody
    • Proteasome subunit, beta type, 5 antibody
    • Proteasome subunit, beta-5 antibody
    • PSB5_HUMAN antibody
    • PSMB5 antibody
    • PSX large multifunctional protease X antibody
    • X antibody
    see all

图片

  • Western blot - Anti-PSMB5/MB1 antibody (ab3330)
    Western blot - Anti-PSMB5/MB1 antibody (ab3330)
    All lanes : Anti-PSMB5/MB1 antibody (ab3330) at 1/1000 dilution

    Lane 1 : NCI-H1299 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
    Lane 3 : Mouse liver cell lysate

    Lysates/proteins at 25 µg per lane.

    Observed band size: 23 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)
    Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)

    Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of A431 (Human epidermoid carcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)
    Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)

    Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of BAEC (Bovine aortic endothelial cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)
    Immunocytochemistry - Anti-PSMB5/MB1 antibody (ab3330)

    Immunocytochemistry/Immunofluorescence analysis of PSMB5/MB1 (green) showing staining in the cytoplasm and nucleus of HeLa (Human epithelial adenocarcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3330 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (30)

发表研究结果有使用 ab3330?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3330 被引用在 30 文献中.

  • Shrum S  et al. The BK activator NS11021 partially protects rat kidneys from cold storage and transplantation-induced mitochondrial and renal injury. Arch Biochem Biophys 688:108410 (2020). PubMed: 32446891
  • Farini A  et al. PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy. Front Physiol 11:403 (2020). PubMed: 32508664
  • Kröll-Hermi A  et al. Proteasome subunit PSMC3 variants cause neurosensory syndrome combining deafness and cataract due to proteotoxic stress. EMBO Mol Med 12:e11861 (2020). PubMed: 32500975
  • Shrum S  et al. Specific BK Channel Activator NS11021 Protects Rat Renal Proximal Tubular Cells from Cold Storage-Induced Mitochondrial Injury In Vitro. Biomolecules 9:N/A (2019). PubMed: 31817165
  • Parajuli N A Cycle of Altered Proteasome and Reactive Oxygen Species Production in Renal Proximal Tubular Cells. Toxicol Forensic Med 4:13-17 (2019). PubMed: 32149269
View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-PSMB5/MB1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Aug 08 2019

Western blot abreview for Anti-PSMB5 / MB1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Gel Running Conditions
Reduced Denaturing (12,5 %)
Loading amount
20 µg
Specification
HEK293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Dr. Melanie Thelen

Verified customer

提交于 May 23 2017

Western blot abreview for Anti-PSMB5/MB1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 breast cancer cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Treatment
Control or PSMB5 siRNA transfected
Specification
MCF7 breast cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jul 14 2010

Western blot abreview for Anti-PSMB5 / MB1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Purified protein (Liver and brain)
Gel Running Conditions
Reduced Denaturing (12,5% SDS gel)
Loading amount
2 µg
Specification
Liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Aug 08 2008

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