重组Anti-PSMB10/MECL1抗体[EPR14902] (ab183506)
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.cn/ps/products/183/ab183506/Images/ab183506-393598-anti-psmb10mecl1-antibody-epr14902-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14902] to PSMB10/MECL1
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
概述
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产品名称
Anti-PSMB10/MECL1抗体[EPR14902]
参阅全部 PSMB10/MECL1 一抗 -
描述
兔单克隆抗体[EPR14902] to PSMB10/MECL1 -
宿主
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, Daudi, HuT 78 and Raji cell lysates; Human fetal spleen and thymus tissue lysates. IHC-P: Human kidney adenocarcinoma tissue. Flow Cyt: Daudi cells.
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常规说明
This product was previously labelled as PSMB10.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14902 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).
|
|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
1/90.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. -
序列相似性
Belongs to the peptidase T1B family. -
发展阶段
Highly expressed in immature dendritic cells (at protein level). -
翻译后修饰
Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5699 Human
- Entrez Gene: 19171 Mouse
- Entrez Gene: 291983 Rat
- Omim: 176847 Human
- SwissProt: P40306 Human
- SwissProt: O35955 Mouse
- SwissProt: Q4KM35 Rat
- Unigene: 9661 Human
see all -
别名
- beta2i antibody
- FLJ00366 antibody
- LMP10 antibody
see all
图片
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Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PSMB10 knockout HeLa cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-4: Merged signal (red and green). Green - ab183506 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab183506 Anti-PSMB10/MECL1 antibody [EPR14902] was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used. Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE. ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.cn/ps/products/183/ab183506/Images/ab183506-215616-anti-psmb10-antibody-epr14902-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.cn/ps/products/183/ab183506/Images/ab183506-215618-anti-psmb10-antibody-epr14902-flow-cytometry.jpg)
Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)Flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.cn/ps/products/183/ab183506/Images/ab183506-215615-anti-psmb10-antibody-epr14902-western-blot.jpg)
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution
Lane 1 : Human fetal spleen tissue lysate
Lane 2 : Human fetal thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa -
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.cn/ps/products/183/ab183506/Images/ab183506-215614-anti-psmb10-antibody-epr14902-western-blot.jpg)
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution
Lane 1 : HuT 78 cell line lysate
Lane 2 : Raji cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa -
![Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.cn/ps/products/183/ab183506/Images/ab183506-6-benefits-of-recombinant-antibodies.png)
Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab183506 被引用在 4 文献中.
- Khilji MS et al. The intermediate proteasome is constitutively expressed in pancreatic beta cells and upregulated by stimulatory, low concentrations of interleukin 1 ß. PLoS One 15:e0222432 (2020). PubMed: 32053590
- Chen C et al. Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. Redox Biol 20:390-401 (2019). PubMed: 30412827
- Huo X et al. Clinical and Expression Significance of AKT1 by Co-expression Network Analysis in Endometrial Cancer. Front Oncol 9:1147 (2019). PubMed: 31781484
- Textor A et al. Preventing tumor escape by targeting a post-proteasomal trimming independent epitope. J Exp Med 213:2333-2348 (2016). PubMed: 27697836