使用敲除细胞株进行验证重组RabMAb

重组Anti-PSMB10/MECL1抗体[EPR14902] (ab183506)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14902] to PSMB10/MECL1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

概述

  • 产品名称

    Anti-PSMB10/MECL1抗体[EPR14902]
    参阅全部 PSMB10/MECL1 一抗

  • 描述

    兔单克隆抗体[EPR14902] to PSMB10/MECL1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human PSMB10/MECL1 aa 50-150. The exact sequence is proprietary.
    Database link: P40306

  • 阳性对照

    • WB: HeLa, Daudi, HuT 78 and Raji cell lysates; Human fetal spleen and thymus tissue lysates. IHC-P: Human kidney adenocarcinoma tissue. Flow Cyt: Daudi cells.

  • 常规说明

    This product was previously labelled as PSMB10.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab183506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

  • 序列相似性

    Belongs to the peptidase T1B family.

  • 发展阶段

    Highly expressed in immature dendritic cells (at protein level).

  • 翻译后修饰

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • beta2i antibody
    • FLJ00366 antibody
    • LMP10 antibody
    • Low molecular mass protein 10 antibody
    • Macropain subunit MECl 1 antibody
    • Macropain subunit MECl-1 antibody
    • MECL1 antibody
    • MGC1665 antibody
    • Multicatalytic endopeptidase complex subunit MECl 1 antibody
    • Multicatalytic endopeptidase complex subunit MECl-1 antibody
    • OTTHUMP00000174858 antibody
    • Proteasome (prosome macropain) subunit beta type 10 antibody
    • Proteasome beta 10 subunit antibody
    • Proteasome catalytic subunit 2i antibody
    • Proteasome MECl 1 antibody
    • Proteasome MECl-1 antibody
    • Proteasome subunit beta 2i antibody
    • Proteasome subunit beta 7i antibody
    • Proteasome subunit beta type 10 antibody
    • Proteasome subunit beta type-10 antibody
    • Proteasome subunit beta-2i antibody
    • Proteasome subunit MECL1 antibody
    • PSB10_HUMAN antibody
    • PSMB 10 antibody
    • Psmb10 antibody
    see all

图片

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PSMB10 knockout HeLa cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab183506 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab183506 Anti-PSMB10/MECL1 antibody [EPR14902] was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used.  Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE.  ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

    Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

    Flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

    Lane 1 : Human fetal spleen tissue lysate
    Lane 2 : Human fetal thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 29 kDa

  • Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution

    Lane 1 : HuT 78 cell line lysate
    Lane 2 : Raji cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 29 kDa

  • Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
    Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)

文献 (4)

发表研究结果有使用 ab183506？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab183506 被引用在 4 文献中.

  • Khilji MS  et al. The intermediate proteasome is constitutively expressed in pancreatic beta cells and upregulated by stimulatory, low concentrations of interleukin 1 ß. PLoS One 15:e0222432 (2020). PubMed: 32053590
  • Chen C  et al. Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. Redox Biol 20:390-401 (2019). PubMed: 30412827
  • Huo X  et al. Clinical and Expression Significance of AKT1 by Co-expression Network Analysis in Endometrial Cancer. Front Oncol 9:1147 (2019). PubMed: 31781484
  • Textor A  et al. Preventing tumor escape by targeting a post-proteasomal trimming independent epitope. J Exp Med 213:2333-2348 (2016). PubMed: 27697836

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