重组Anti-PSMA3抗体[EPR5455] (ab109532)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5455] to PSMA3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-PSMA3抗体[EPR5455]
参阅全部 PSMA3 一抗 -
描述
兔单克隆抗体[EPR5455] to PSMA3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- A549, JAR, PC12 and NIH3T3 cell lysates; Human brain tissue; HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5455 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109532于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
|
ICC/IF | (1) |
1/100 - 1/250.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
-
功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2. -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 5684 Human
- Entrez Gene: 19167 Mouse
- Entrez Gene: 29670 Rat
- Entrez Gene: 408248 Rat
- Omim: 176843 Human
- SwissProt: P25788 Human
- SwissProt: O70435 Mouse
- SwissProt: P18422 Rat
see all -
别名
- HC8 antibody
- Macropain subunit C8 antibody
- MGC12306 antibody
see all
图片
-
All lanes : Anti-PSMA3 antibody [EPR5455] (ab109532) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : JAR cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa -
ab109532, at 1/100 dilution, staining PSMA3 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
ab109532, at 1/100 dilution, staining PSMA3 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (7)
ab109532 被引用在 7 文献中.
- Liu J et al. Immuno-oncological role of 20S proteasome alpha-subunit 3 in aggravating the progression of esophageal squamous cell carcinoma. Eur J Immunol 52:338-351 (2022). PubMed: 34755333
- Liu Q et al. The IFN-?-induced immunoproteasome is suppressed in highly pathogenic porcine reproductive and respiratory syndrome virus-infected alveolar macrophages. Vet Immunol Immunopathol 226:110069 (2020). PubMed: 32535163
- Takahashi M et al. USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis. iScience 9:433-450 (2018). PubMed: 30469013
- Moritz KE et al. The role of the immunoproteasome in interferon-?-mediated microglial activation. Sci Rep 7:9365 (2017). PubMed: 28839214
- Cirigliano SM et al. The synthetic peptide CIGB-300 modulates CK2-dependent signaling pathways affecting the survival and chemoresistance of non-small cell lung cancer cell lines. Cancer Cell Int 17:42 (2017). ICC/IF ; Human . PubMed: 28373828
- Li Y et al. Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths. Sci Rep 7:40698 (2017). WB ; Chicken . PubMed: 28079188
- Geng S et al. Protein Interaction between Ameloblastin and Proteasome Subunit a Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel. J Biol Chem 290:20661-73 (2015). PubMed: 26070558