Application
Western blot
Sample
Human Tissue lysate - whole (Brain, frontal cortex)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
10 µg
Specification
Brain, frontal cortex
Blocking step
LiCor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: LiCor blocking buffer
Dilution
1/10000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: LiCor
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Detection
Detection method
LiCor Laser
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: 100 kDa
Additional data
Additional Notes
Molecular weight of markers are from top to bottom (major bands) 200kDa, 110kDa, 60kDa. Lane 1 and 2 are 10 ug of human brain homogenate.
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提交于 Jun 16 2009