Anti-PSD95抗体[6G6-1C9] - Synaptic Marker (ab2723)

Reviews (14)Q&A (3)References (111)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
  • Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
  • Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)

Key features and details

  • Mouse monoclonal [6G6-1C9] to PSD95 - Synaptic Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-PSD95抗体[6G6-1C9] - Synaptic Marker
    参阅全部 PSD95 一抗

  • 描述

    小鼠单克隆抗体[6G6-1C9] to PSD95 - Synaptic Marker

  • 宿主

    Mouse

  • 特异性

    We do not guarantee IHC-P for mouse.

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data

  • 免疫原

    Recombinant full length protein corresponding to Rat PSD95.

  • 阳性对照

    • IHC-P: Rat retina tissue. WB: Rat and mouse hippocampus and forebrain tissue lysate.

  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Rat
WB
Rat
All applications
Cow
Zebrafish
应用 Ab评论 说明
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse.
WB (7)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
说明
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).

靶标

  • 功能

    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

  • 组织特异性

    Brain.

  • 序列相似性

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.

  • 结构域

    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

  • 翻译后修饰

    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

  • 细胞定位

    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)

    IHC image of ab2723 staining in formalin-fixed, paraffin-embedded rat retinal tissue, performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2723, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    Secondary-only control image is shown as insert.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    All lanes : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 5 µg/ml

    Lane 1 : Hippocampus (Rat) Tissue Lysate
    Lane 2 : Forebrain (Rat) Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Forebrain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 110 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    Western blot - Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)Image courtesy of an AbReview submitted by Grant Corbett
    Lanes 1-6 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution
    Lanes 7-12 : Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723) at 1/1200 dilution (18 hours at 4°C)

    Lanes 1-6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lanes 7-12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size: 80 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes

    See Abreview

文献 (111)

发表研究结果有使用 ab2723？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2723 被引用在 111 文献中.

  • Lundgren JL  et al. Proximity ligation assay reveals both pre- and postsynaptic localization of the APP-processing enzymes ADAM10 and BACE1 in rat and human adult brain. BMC Neurosci 21:6 (2020). PubMed: 32019490
  • Post KL  et al. Multi-model functionalization of disease-associated PTEN missense mutations identifies multiple molecular mechanisms underlying protein dysfunction. Nat Commun 11:2073 (2020). PubMed: 32350270
  • Wu Q  et al. SRC-1 Knockout Exerts No Effect on Amyloid ß Deposition in APP/PS1 Mice. Front Aging Neurosci 12:145 (2020). PubMed: 32625077
  • Feng C  et al. Calcium-Sensing Receptor Mediates ß-Amyloid-Induced Synaptic Formation Impairment and Cognitive Deficits via Regulation of Cytosolic Phospholipase A2/Prostaglandin E2 Metabolic Pathway. Front Aging Neurosci 12:144 (2020). PubMed: 32670047
  • Sun T  et al. Anxiolytic Effects of 8-O-Acetyl Shanzhiside Methylester on Acute and Chronic Anxiety via Inflammatory Response Inhibition and Excitatory/Inhibitory Transmission Imbalance. Neurotox Res N/A:N/A (2020). PubMed: 32367473
View all Publications for this product

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Frontal cortex)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Frontal cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Read More

Dr. Alexandre Magno

Verified customer

提交于 Jun 26 2019

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse brain primary culture)
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (NuPAGE 4-12% Bis-Tris precast protein gel))
Loading amount
400000 cells
Specification
Mouse brain primary culture
Blocking step
Li-Cor Odyssey blocking buffer as blocking agent as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 28°C
Read More

Abcam user community

Verified customer

提交于 Jan 10 2019

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus and Liver)
Gel Running Conditions
Reduced Denaturing (8% in TGS)
Loading amount
30 µg
Specification
Hippocampus and Liver
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Read More

Grant Corbett

Verified customer

提交于 Jul 17 2014

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse hippocampus and cerebellum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse hippocampus and cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Sep 19 2013

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Brain Homogenate)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Brain Homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Mar 14 2012

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions
Reduced Denaturing (7.5% SDS-PAGE)
Loading amount
10 µg
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Sep 09 2010

Western blot abreview for Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain, frontal cortex)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
10 µg
Specification
Brain, frontal cortex
Blocking step
LiCor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jun 16 2009

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